Publication: Antimicrobial enhancement of denture base resin using microcapsule drug delivery technology: a characterization study
| dc.contributor.author | Mahadi, Nik Nur Syahidatul Jannah | |
| dc.date.accessioned | 2025-10-26T04:47:05Z | |
| dc.date.available | 2025-10-26T04:47:05Z | |
| dc.date.issued | 2025-05 | |
| dc.description.abstract | Denture stomatitis (DS) presents as a common inflammatory reaction in denture wearing patients. It is highlighted that the rising demand for dentures, particularly among the elderly, due to factors such as aging populations and associated health issues affecting oral hygiene. Alternatively, new development of antimicrobial elimination that could effectively inhibit the growth of oral bacteria could be an active area of research. The aim of this study is to investigate the antimicrobial interaction of PLA/TTO microcapsules in polymerized PMMA denture base resin against Staphylococcus aureus, Streptococcus mutans and Candida albicans. A new drug delivery methodology employed via emulsion and solvent evaporation (ESE) techniques was performed to synthesize polylactic acid (PLA) microcapsules containing antimicrobial agents; tea tree oil (TTO). The characterization of TTO/PLA microcapsules were characterized using Ultraviolet-visible (UV-Vis), Fourier-transform Infrared (FTIR), Gas chromatography-mass spectrometry (GCMS) and field emission scanning electron microscope (FESEM). The modified PMMA denture base was prepared in three different concentrations of prepared microcapsules (TTO: PLA (% w/w)), 10%, 50% and 100%. For antimicrobial activity, the modified PMMA was evaluated for antimicrobial activity through leaching solution at the intervals of 7th, 14th and 30th days using the well diffusion test and time-killed assay. Then, FESEM images was performed to assess the attachment of microorganism on the surface of modified denture base. The results obtained from characterization analysis showed the microcapsules were successfully synthesized using emulsion and solvent evaporation method where the drug release study and the average particle size formed is homogenously distributed for controlled drug release. The antimicrobial activity of modified PMMA base resin incorporated with PLA/TTO microcapsules showed a good antimicrobial effect (Table 4.2/4.3/4.4/4.5) and statistically revealed a significant effect (p<0.05) against S. aureus and C. albicans where the inhibition area increased as the concentration increased except for S. mutans. The time killed assay was used to study the antimicrobial agent over the time, it showed that the antimicrobial activity of modified denture base exhibited a good antimicrobial effect against all microorganism tested. In morphological test using FESEM, the attachment of fungal and bacteria cell tested is lesser as the concentration increased within 24 hours studied. The study revealed incorporation of PLA/TTO microcapsules in denture base, has significant impacted on enhanced antimicrobial activity, offering potential usefulness for denture users in the future. However, further studies including physical properties and cytotoxicity are needed to be investigated before the clinical trials. | |
| dc.identifier.uri | https://erepo.usm.my/handle/123456789/22921 | |
| dc.language.iso | en | |
| dc.title | Antimicrobial enhancement of denture base resin using microcapsule drug delivery technology: a characterization study | |
| dc.type | Resource Types::text::thesis::master thesis | |
| dspace.entity.type | Publication | |
| oairecerif.author.affiliation | Universiti Sains Malaysia |