Parameters that effect the antioxidant profile and phenolic compound from centella asiatica extract
Loading...
Date
2018-06
Authors
Muhammad Ridhwaan Mohd Bakhtiar
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Centella asiatica is commonly used as a spice and medicinal herb due to its
antioxidant activity and total phenolic contents. Water, methanol and ethanol were used
to analyze the amount of antioxidant activity and total phenolic contents. Ethanol
extraction at 7 hr showed the highest value of DPPH scavenging activity with 65.06 %
(IC50=291.00 mg/ml) followed by water with 63.48 % (IC50=408.26 mg/ml) and
methanol with 62.39 % (IC50=515.86 mg/ml). The data from the experiment had been
compared with the BHT standard curve in order to finalised the best solvent based on
DPPH scavenging activity. The increase in DPPH scavenging activity contributes by
high amount of TPC. Ethanol gave the highest TPC followed by water and methanol at
228.57 mgGAE/g, 115.63 mgGAE/g and 64.41 mgGAE/g, respectively. This is because
ethanol has stronger hydroxyl group compared to methanol and water. The results were
compared with the standard curve of gallic acid. As the time increase the phenolic
compound from C.asiatica also increase, this is because the phenolic content
contributed by capacity of extract due to the time of extraction. The active component
that contributed to the phenolic compound is asiaticoside. As The retention time using
HPLC was 2.60 min while the standard time was 2.75 min. The result showed that
ethanol is the best solvent for extraction of active component with antioxidant activity
and phenolic contents of C. asiatica compared to methanol and water.