Synthesis Of Silver Nanoparticles Via Pulse Laser Ablation In Liquid For Anti-Bacteria Applications
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Date
2018-01
Authors
Shanon, Mayasa Abdulwahid
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
Various types of bacteria affected the human health and cause different diseases like skin infection nasal passage, food poisoning, etc. These bacteria acquired immunity and develop self-resistance against some familiar antibiotics. Therefore, considerable demand is emerged on nobel metal nanoparticles which have ability to eliminate those types of bacteria. Q – Switched Nd:YAG laser pulse of 10 ns was employed to produce colloidal suspension of silver nanoparticles by laser ablation in distilled water. This technique provides controllable process for size, size distribution and shape of nanoparticles. Effects of various laser parameters were examined on the ablation efficiency such as laser wavelength, laser energy density and number of pulses. Silver nanoparticles produced in this work were characterized using morphological investigation (HRTEM and AFM) and spectroscopic method (UV-Vis absorption). The experimental data were fitted with Mie-Gans theoretical model to estimate the nanoparticles size and their shape. The prepared silver nanoparticles were tested on some famous types of bacteria live in human body such as Staphylococcus, Streptococcus, Proteus and E. coli. It is found that the fundamental laser wavelength of 1064 nm could produce high density of silver nanoparticles with larger size of 100 nm and wider size distribution. While smaller nanoparticles of average diameter of 70 nm were synthesized by the second harmonic generation of 532 nm wavelength. Our results reveal formation of controllable shapes via using proper laser parameters. The silver nanoparticles features were found very effective on the antibacterial activity against the bacteria. Silver nanoparticles of mean diameter 75 nm was obtained using laser energy of 600 mJ, while uniform silver nanoparticles size distribution can be obtained with minimum mean diameter of 20 nm was achieved by laser energy density of 20 J/cm2. It is found that the antibacterial activity of silver nanoparticles depends on the size and shape of nanoparticles.
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Keywords
Silver nanoparticles via pulse laser ablation , in liquid for anti-bacteria applications