Quaternary Aqueous Biphasic Mixture For Protein Extraction From Papaya
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Date
2022-07-01
Authors
Loh, Li Sin
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
The aqueous biphasic system is a liquid-liquid separation method which has gained great interest for the extraction of enzymes, nucleic acids, and bioactive compounds. This is because ABS-based DES has the properties of being easy to scale up, environmentally friendly, and providing a high recovery yield. However, the partition behaviour involved is complex and difficult to predict. In this research, different parameters were investigated for the formation of ABS-based DES. Four different types of DES were synthesized including ChCl:U, ChCl:Gly, ChCl:Glu, ChCl:Ala, and ChCl:Lac at different molar ratios. ABS was synthesised using different types of salt, including Na2SO4, KH2PO4, and (NH4)2SO4 with PEG2000. Single factor experiments demonstrated that the formation of ABS was affected by the types of salt, the concentration of salt solutions, concentration of PEG, types of hydrogen bond donors in DES synthesized and the volume of DES. The results obtained demonstrated a negative correlation between the concentration of salt solutions and the phase-volume ratio, whereas the concentration of PEG and the volume of DES were positively correlated. The concentration of salt solutions and volume of DES were positively correlated with the rate of formation of ABS, whereas the concentration of PEG was negatively correlated. The maximum yield of protein extracted (8456.5667g/mL) from papaya extract crude was attained under the following optimal extraction conditions: 0.3M of PEG2000 and 1.0M of Na2SO4, where the volume ratio was 1:1, and ChCl:Lac DES with a molar ratio of 1:2, where the volume ratio of ABS and DES was 10:1. The maximum protein extraction efficiency obtained was 90.03%, with the highest partition coefficient of 3.6119.