Fabrication and characterisation of gelatine- hydroxyapatite (hap) porous scaffold
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Date
2016-06
Authors
Ho Hong Hui
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Abstract
In this research, Hydroxyapatite (HAp) scaffolds coated with gelatine are fabricated via polymer reticulate method and Polyurethane foam (PU) are used as the scaffold template. The HAp powders used was first calcined at 1100 °C able reduce viscosity and agglomeration effect. Slurry with 40wt% solid loading of calcined HAp powders and additional 3 wt% of Polyvinyl alcohol (PVA) powder was prepared, then immerse the PU foam into the slurry. Heat treatment started with burnout PU foam at 650 °C for an hour followed by sintering up to 1100 °C and soak for another one hour. Lastly, the Gelatine/HAp scaffold was coated of gelatine. The effect of gelatine concentration and crosslinking temperature on the compressive strength were studied. The average compressive strength for 10 wt%, 15 wt% and 20 wt% gelatine coated HAp scaffold are of 7.8 kPa, 22.8 kPa and 71.0 kPa respectively. On the other hand, the average compressive strength for crosslinking temperature treated at 130 °C and 160 °C are 66.4 kPa and 72.3 kPa. XRD analysis had confirmed that partial HAp had transformed into beta-tricalcium phosphate (β-TCP). After calcination of HAp powder, 66 % HAp transformed into β-TCP, while after sintering of HAp scaffold, 71 % of β-TCP found. FTIR and EDX analysis shown the higher amount of gelatine content found in scaffolds with higher crosslinking temperature and higher wt% of gelatine used together with increased in compressive strength. SEM analysis also revealed number of morphology which contribute to the low strength properties of these scaffolds.