Production Of Novel Recombinant Antipfhrp2 Vnar-G1 Protein Using Escherichia Coli Bl21(De3) Expression System

Simple item page
dc.contributor.authorKok, Boon Hui
dc.date.accessioned2021-07-28T01:44:21Z
dc.date.available2021-07-28T01:44:21Z
dc.date.issued2020-06
dc.description.abstractMalaria rapid diagnostic tests (RDTs) act as important antibody-based immunoassays for prompt malaria diagnosis. Conventional monoclonal antibodies (mAbs) are widely used in RDTs but it can be easily degraded at high ambient temperatures. Hence, the shark VNARS might be good alternatives to mAbs due to its higher thermal stability and binding affinity with antigens. In this study, the recombinant anti-PfHRP2 VNAR-G1 protein was produced in E. coli expression system through various steps such as recombinant cell isolation, PCR, agarose gel electrophoresis, plasmid extraction, transformation and protein expression. Besides, the combinatorial effects of temperature and IPTG concentration towards the cell density of recombinant BL21(DE3) based on the absorbance readings and cell wet weights were investigated using software R.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/13850
dc.language.isoenen_US
dc.publisherUniversiti Sains Malaysiaen_US
dc.subjectProduction Of Novel Recombinanten_US
dc.subjectAntipfhrp2 Vnar-G1 Proteinen_US
dc.subjectEscherichia Coli Bl21(De3)en_US
dc.subjectExpression Systemen_US
dc.titleProduction Of Novel Recombinant Antipfhrp2 Vnar-G1 Protein Using Escherichia Coli Bl21(De3) Expression Systemen_US
dc.typeOtheren_US
Files
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Pusat Pengajian Teknologi Industri - Monograf