Study On The Effects Of Blue And Green Laser Beams On Anaemic Blood Parameters
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Date
2015-10
Authors
Suardi, Nursakinah
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Abstract
For the past few decades, a lot of experimental has shown positive response on bio-stimulation after laser irradiation on living bodies such as wound healing, tissue repair, and therapeutic treatment. In this study, human blood parameter from normal and anaemic respondents which consists of white blood cell, red blood cell, haematocrit, mean cell volume, mean corpuscular haemoglobin and platelet were analysed on the effects of low level laser irradiation using DPSS green laser (532 nm) and diode blue laser (460 nm). Objectives of this research are to study biological response of low intensity laser on normal and anaemic red blood cell before and after irradiate with laser, to study cell survival or viability of the blood cell before and after irradiation, to study morphological properties of blood based on different laser parameters and assessing the role of low intensity laser wavelength in improving and enhancing anaemic red blood cell. Irradiation of blood samples with green and blue lasers showed significant changes in red blood cell, haematocrit, mean cell volume and platelet with no significant changes correlated with gender and age except platelet. Blood category has showed significant changes in haematocrit after irradiation with both blue and green laser. When comparing the effect of both lasers on blood parameter, white blood cell and platelet parameter show significant difference among laser types. The difference in blood parameter between both lasers does not depend on respondent’s gender but difference in platelet does depend on respondent’s age. Further, investigation on red blood cell viability for normal and anaemic sample shows significant differences on red blood cell viability among exposure time after laser irradiation. From the analysis, it was revealed that there is significant difference between cell viability among laser type. Blue laser irradiation affected most on cell viability compared to green laser irradiation. Finally, study of morphology of red blood cells before and after laser irradiation caused deformability to red blood cell. Green laser irradiation affected the shape of red blood cell more compared to blue laser. Anaemic blood samples morphology affected most by laser irradiation compared to normal blood samples and green laser affected red blood cell morphology more compared to blue laser. From all the blood smears prepared, most of the abnormal cell observed is echinocyte which is crenated cell compared to other red blood cell variations. As a conclusion, for desired therapeutic effect on anaemic patient using low level intensity laser, blue laser considered safer at 30 – 60 s as less damaging effect were observed compared to green laser. After 60 s, more damaging effect was observed on red blood cell parameter. Thus, 60 s should be a cut point if using blue laser.
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Physics (General)