Spion-C595 Nanoprobe For Magnetic Resonance Image Contrast Enhancement Of Hormone Dependent Breast Cancer Cells
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Date
2016-09
Authors
Khaniabadi, Pegah Moradi
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Abstract
Currently, effective and specific diagnostic imaging of breast cancer in early stages is a major challenge. Nanomedicine plays an essential role by delivering the contrast agent in a targeted manner to specific tumor cells, leading to improvement in accurate diagnostic by good visualization and specific demonstration of tumor cells. This study investigated the fabrication, characterization and application (in vitro and in vivo) of a specific breast cancer MR contrast agent. C595 monoclonal antibody-conjugated superparamagnetic iron oxide nanoparticles (SPION-C595) was developed by EDC method, for early stage breast tumor detection with MUC1 over-expression. Moreover, monoclonal antibody conjugation on superparamagnetic iron oxide was confirmed using FTIR, XRD, TEM, SEM-EDAX and zetasizer techniques. More than 84% and 98% signal enhancement was observed for T1 and T2 weighted images, respectively at doses of 100 μg Fe/ml of SPION-C595. To evaluate the efficacy of SPION-C595 several in vitro studies were conducted. In vitro cell cytotoxicity studies were conducted on the number of viable MCF 7 cells. It was found that SPION-C595 did not exhibit significant toxicity. Furthermore, prolonged incubation of the MCF 7 cells with SPION-C595 did not affect the cell morphology and its proliferation ability. Selectivity of the nanoprobe for this type of breast cancer is observed on MCF 7 cells by
Prussian blue and AAS. Moreover, 3D solid tumor Prussian blue, a novel method was established to demonstrate the binding of the nanoprobe on solid tumor in vitro. The results show significant binding of 200 μg Fe/ml nanoprobe towards MCF 7 breast tumor. The MR in vitro imaging shows the obvious change of T2-weighed images at a 76% reduction compared with untreated cells at doses of 200 μg Fe/ml. Moreover, MR in vivo images shows significant enhancement of T1 and T2 relaxation times of breast tumor after administration of SPION-C595. Significant contrast enhancement of breast cancer could still be clearly seen even 24 hours post-injection. A significant tumor uptake was observed in solid breast in the tested animals. It thus can be concluded that the magnetic nanoparticles conjugated with C595 exhibit high dual (T1 and T2) MR contrast potential, and can be applied as specific breast cancer contrast agent.
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The fabrication, characterization and application , of a specific breast cancer MR contrast agent.