Effect of high temperature toward microalgal organic matter

dc.contributor.authorHarsimran Kaur Kler A/P Lakbir Singh
dc.date.accessioned2021-03-04T07:46:55Z
dc.date.available2021-03-04T07:46:55Z
dc.date.issued2019-06
dc.description.abstractAlgal blooms can seriously affect the operation of water treatment especially membrane treatment processes mainly due to accumulation of algal organic matter (AOM). Studies have shown that AOM is capable of causing severe fouling in low pressure membrane processes. In the recent years, membrane distillation (MD) has gained significant regard in the industry for water treatment purpose. However, fouling in MD differs from pressure-driven membrane processes mainly due to the higher temperature used in MD process. In this study, the different components of AOM, which are soluble extracellular polymeric substance (EPS), bounded EPS and internal organic matter (IOM) were extracted from three common species of benthic algae (Amphora coffeaeformis, Cylindrotheca closterium and Navicula salinarum) and they were characterized for the concentration of carbohydrate, the molecular weight (MW) distribution of carbohydrate and the concentration of protein. These characterization was done by employing colorimetric analysis, such as phenol-sulphuric acid method to measure the carbohydrate concentration and bicinchoninic acid (BCA) method to determine the protein concentration. The characterization of the AOM was done after the algal were harvested at room temperature, and also after they were exposed to higher temperature at 60oC, 70oC and 80oC for 1 hour. This is to compare the characteristics of the AOM at normal conditions and also at higher temperature during MD process. The EPS produced by the three algal species comprised of higher concentration of carbohydrate compared to protein, where there is 93.04 % of carbohydrate for A. coffeaeformis diatoms, 78.35 % for N. salinarum diatoms and 82.83 % of carbohydrate for C. closterium diatoms. On the other hand, the protein percentage in A. coffeaeformis diatoms was 51.77 %, for C. closterium diatoms was 40.97%, and for N. salinarum diatoms was 72.25%. Hence, the carbohydrate and protein content varies in different species, and cannot be generalized for every algal species. When the temperature exposed to the algal cells were increased, it was seen that the concentration of carbohydrate and protein in EPS increases, while the concentration of carbohydrate and protein in IOM decreases. Apart from that, it was found that the composition of higher MW of carbohydrate in AOM decreases at higher temperature, while the composition of lower MW of carbohydrate in AOM increases at higher temperature. Overall, the compositions of carbohydrate, protein and the MW distributions in the soluble EPS, bounded EPS and IOM at different temperatures will likely dictate the severity of fouling in MD during algal blooms.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/11784
dc.language.isoenen_US
dc.titleEffect of high temperature toward microalgal organic matteren_US
dc.typeOtheren_US
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