Synthesis Of Methyl Acetate By Lipase Catalyzed Esterification
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Date
2021-06-01
Authors
Izudin @ Mohamad, Muhammad Firdaus
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
The beginning of esterification process is mostly by using chemical synthesis with the
help of chemical catalyst which often involved binary or tertiary azeotropic mixture which
lead to difficulty in producing high purity of product and environmental issues. Hence,
esterification with the aid of biocatalyst was introduced which is proven to have a better
potential especially with lipase in producing esters through enzymatic esterification. In this
study, methyl acetate which has high market demand due to its end uses was synthesise by
direct esterification reaction catalysed by immobilized lipase from Candida rugosa by using
n-hexane as organic solvent in batch system. Celite-545 was used as a support material on
immobilized Candida rugosa lipase with catalytic activity of 0.6833 U/mg.
One-Factor-at-One-Time (OFAT) method was studied in order to identify the effect
of the optimizing parameters including reaction time, enzyme loading, agitation speed and
substrate molar ratio of acetic acid and methanol. The optimal acid conversion for methyl
acetate was 58% obtained at 2 hours incubation time with 10 U/ml of enzyme loading at 45
and 150 rpm of agitation speed with a substrate molar ratio of 1:3 (acetic acid to methanol).
The comparison between kinetic models of enzyme catalysed esterification with the
secondary data from the article was also performed by using non-linear regression analysis.