A Preliminary Study: Protein Extraction Of Migratory Locust (Locusta Migratoria) Via Alkaline Isoelectric Precipitation Method
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Date
2021-06
Authors
Nurul Sofea Asrul Hadi
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
Unprecedented growth of future human population around the globe seems
non capable to overcome high food demand of animal livestock. Increasing in
population will coincidentally shrink more livestock farming land. Hence,
entomophagy or known as eating insect practice is a promising alternative to
circumvent the issue by utilizing the insect protein in worldwide food application. In
this preliminary study, protein extraction of oriental migratory locust (Locusta
migratoria manilensis) using alkaline isoelectric precipitation was investigated.
Firstly, the effect of lipid removal technique to protein content was investigated.
Secondly, pH 9 to 13 during solubilisation was applied to obtain the highest protein
yield and extraction rate. Initially, the whole dried locust was ground and defatted
using ethanol solvent. Defatted locust (DLM) was subjected to alkaline solubilisation
for 60 min at 40℃ with addition of 0.25 M NaOH and pH adjustion if necessary.
Description
Keywords
Protein Extraction Of Migratory Locust , A Preliminary Study , Via Alkaline Isoelectric Precipitation Method