Morphological, pathogenicity and molecular studies of fusarium isolates from orchids

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dc.contributor.authorMohamad Zain, Nurhayati
dc.date.accessioned2014-11-14T02:33:23Z
dc.date.available2014-11-14T02:33:23Z
dc.date.issued2007
dc.descriptionMasteren_US
dc.description.abstractA total of 32 Fusarium spp were isolated from Dendrobium (29 isolates) and Oncidium (3 isolates) showing symptoms of root and stem rot. Another eight isolates were obtained from Fusarium stock cultures of USM. Based on morphological characteristics, the isolates were identified as F. oxysporum, F. proliferatum and F. solani. Pathogenicity tests against F. oxysporum, F. proliferatum and F. solani were conducted on healthy Dendrobium and the results showed that the three Fusarium were pathogenic to Dendrobium. In VCG analysis, nitrate-nonutilizing mutants were generated from all F. proliferatum and F. oxysporum isolates on a medium amended with maximum 2.5% KClO3. Nit mutant was not generated by F. solani. Four and five vegetative compatibility groups were identified from F. proliferatum and F. oxysporum, respectively. For molecular analysis using RAPD and PCR-RFLP of ITS1-5.8S-ITS2 regions, banding patterns generated were able to distinguish all the three species causing root and stem rot on Dendrobium and Oncidium. Based on UPGMA cluster analysis, all the three species were clustered in a different clusters. Therefore, the present study showed that morphological characteristics and molecular techniques of RAPD and PCR-RFLP of ITS1- 5.8S-ITS2 can be used to characterize Fusarium species from Dendrobium and Oncidium orchids.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/452
dc.language.isoenen_US
dc.subjectScience biologyen_US
dc.subjectfusarium isolatesen_US
dc.subjectorchidsen_US
dc.titleMorphological, pathogenicity and molecular studies of fusarium isolates from orchidsen_US
dc.typeThesisen_US
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