Synthesis Of Ethyl Oleate Catalyzed By Immobilized Lipase Process Optimization
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Date
2022-06-01
Authors
Low, Hui Yen
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
Esterification synthesis by chemical synthesis, using strong acid as the catalyst causes drawbacks to the environment, formation of undesirable side products and the presence of high acidic conditions. Hence, esterification using lipase as biocatalysts is considered a promising alternative to produce ester through enzymatic esterification, because this method is more eco-friendly, as it proceeds at mild reaction conditions, thus reducing energy consumption and adverse environmental effects. In this study, ethyl oleate, a commercially valuable oleate ester, was synthesised by direct esterification reaction catalysed by immobilized lipase from Candida rugosa in batch system using n-hexane as organic solvent. The free C. rugosa lipase was successfully immobilised on functionalized multiwalled carbon nanotubes support.
The influence of optimizing parameters including reaction time, enzyme loading, temperature, acid:ethanol molar ratio were studied using one-factor-at-a time (OFAT) method. Optimal conversion yield for ethyl oleate of 87% was obtained at 40 minutes incubation time with 30 U/ml of enzyme loading at 40℃ with a substrate molar ratio of 1:3 (oleic acid to ethanol).
The comparison between bisubstrate kinetic models of enzyme catalysed esterification by correlating experimental findings from reported research study was also investigated using non-linear regression analysis. A model of Ping Pong Bi Bi with inhibition by both substrates was found to exhibit the best fit with the experimental value of where the model parameters were obtained as Vmax=11.93 mmol mg−1min−1 , Km,A=3.02×10−3 mmol L−1,Km,B=3.04×10−3 mmol L−1,Ki,A=6.94mmol L−1, Ki,B=1.14mmol L−1 which shows good agreement to the reported findings.