Development of a Technique to Determine Blood Hemolysis Due to Longer Storage at Various Visible Light Wavelengths
| dc.contributor.author | Yahaya Bermakai, Madhiyah | |
| dc.date.accessioned | 2016-10-24T04:19:06Z | |
| dc.date.available | 2016-10-24T04:19:06Z | |
| dc.date.issued | 2010 | |
| dc.description.abstract | Standard clinical laboratory method for determining blood hemolysis is to spin the sample in centrifuge and then, visually examine the plasma fraction being contaminated or not. Currently, there is no practical method for determining whether a sample is hemolyzed which operates on whole-blood. The purpose of this research is to develop a technique to determine blood hemolysis due to longer storage at various visible light wavelengths and also to study the factors that affect the patterns of light absorption and transmission caused due to the hemolysis. Various types of ABO blood group samples were taken from 100 patients in the USM Health Centre. Eight different light sources with wavelengths in the range of 300 nm to 700 nm were used to irradiate the. Intensity readings were detected through the blood and the count of the blood cells were also acquired for 25 consecutive days using the automated hematology analyzer. The finding shows that concentration of free hemoglobin plasma and red blood cells derivatives play an important role in absorbance and transmittance of visible light wavelengths, thus, indicated the level of hemolysis in the whole-blood sample. This research is useful in determining whether the blood sample is hemolyzed and also can indicate the level of the hemolysis when tested on the whole-blood. Furthermore, this technique can be used where a centrifuge is not available. | en_US |
| dc.identifier.uri | http://hdl.handle.net/123456789/2834 | |
| dc.subject | Blood Hemolysis | en_US |
| dc.subject | Light Wavelengths | en_US |
| dc.title | Development of a Technique to Determine Blood Hemolysis Due to Longer Storage at Various Visible Light Wavelengths | en_US |
| dc.type | Thesis | en_US |
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