Deposition of nylon membrane on carbon electrode for the development of ascorbic acid electrochemical biosensor
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Date
2018-06
Authors
Noor Nadhirah Harith
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Abstract
Electrochemical biosensor is the combination of electroanalytical methods of high
sensitivity with a presence of biorecognition system that helps in identifying target
species. The main objective of this work is to study the conjugation of ascorbic acid (AA)
to polyaniline (PANI) and to explore the cyclic voltammetry and electrochemical
impedance spectroscopy of the membrane-based sensor to detect AA. In this study, the
conjugation of ascorbic AA was first done by the synthesis of PANI/Fe2O3, before the
AA was interacted with PANI/Fe2O3. UV-Vis spectrometer absorbance for solutions
PBS, AA of 50 mM, PANI, PANI/Fe2O3 and PANI/Fe2O3/AA of 50 mM were measured
to prove the successful conjugation of AA. Next, membrane-sensor was assembled, bare
glass carbon electrode (GCE), GCE-nylon membrane and GCE-nylon membrane
immobilized with ascorbate oxidase were testing with AA. The cyclic voltammetry
pattern and electrochemical impedance spectroscopy were recorded to study the redox
potential and conductivity of the testing solutions.