Development of rhizobial inoculant for leguminous cover crop Mucuna bracteata using local kaolin and rice husk as carriers.

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dc.contributor.authorAhmad Ghazali, Amir Hamzah
dc.date.accessioned2017-07-03T07:01:36Z
dc.date.available2017-07-03T07:01:36Z
dc.date.issued2013
dc.description.abstractOur aim of this project is to increase the ability of the legume cover crop Mucuna bracteata to serve as a nitrogen source for oil palm, by establishing effective relationships with diazotrophic rhizobacteria. We have successfully isolated and identified non-rhizobial diazotrophic microsymbionts from root nodules of M. bracteata and it provides details for their potential use with Mucuna. Our findings indicated that the isolated microsymbionts could nodulate and promote N:cfixation activity and enhanced growth in association with the host plants. The 16S rONA sequencing revealed that the diazotrophic microsymbionts consisted of A/phaproteobacteria, Betaproteobacteria and Gammaproteobacteria classes of rhizobacteria. The study continues on survival assessment of the rhizobia (Burkholderia sp. USM B20) in various carrier materials. The results showed that carrier peat stored at 280C was able to sustain the highest viable cell number of rhizobia. Optimization processes of the rhizobial biomass were also performed in a shake flask experiment and subsequently upscale it into bioreactor. Overall, we believe that proper inoculation of Mucuna with bioinoculants will reduce dependence on chemical fertilizer inputs. It helps to increase the productivity and environment-friendly practices of Malaysian oil palm producers.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/4075
dc.subjectRhizobial inoculanten_US
dc.subjectBiofertilizeren_US
dc.subjectSustainable Agricultural Practicesen_US
dc.subjectBurkholderia sp.en_US
dc.subjectMucuna bracteataen_US
dc.subjectLeguminous cover cropen_US
dc.subjectBiomass Optimizationen_US
dc.titleDevelopment of rhizobial inoculant for leguminous cover crop Mucuna bracteata using local kaolin and rice husk as carriers.en_US
dc.typeTechnical Reporten_US
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