Production Of Novel Recombinant Antipfhrp2 VNAR-G1 Protein Using Escherichia Coli Bl21(DE3) Expression System

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dc.contributor.authorKok, Boon Hui
dc.date.accessioned2021-07-23T06:15:45Z
dc.date.available2021-07-23T06:15:45Z
dc.date.issued2020-06
dc.description.abstractMalaria rapid diagnostic tests (RDTs) act as important antibody-based immunoassays for prompt malaria diagnosis. Conventional monoclonal antibodies (mAbs) are widely used in RDTs but it can be easily degraded at high ambient temperatures. Hence, the shark VNARS might be good alternatives to mAbs due to its higher thermal stability and binding affinity with antigens. In this study, the recombinant anti- PfHRP2 VNAR-G1 protein was produced in E. coli expression system through various steps such as recombinant cell isolation, PCR, agarose gel electrophoresis, plasmidextraction, transformation and protein expression.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/13835
dc.language.isoenen_US
dc.publisherUniversiti Sains Malaysiaen_US
dc.subjectProduction Of Novel Recombinanten_US
dc.subjectAntipfhrp2 VNAR-G1 Proteinen_US
dc.subjectEscherichia Colien_US
dc.subjectBl21(DE3)en_US
dc.subjectExpression Systemen_US
dc.titleProduction Of Novel Recombinant Antipfhrp2 VNAR-G1 Protein Using Escherichia Coli Bl21(DE3) Expression Systemen_US
dc.typeOtheren_US
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