Innovative Approaches Towards Development And Utilization Of Dna Diagnostics For Salmonella Typhi
| dc.contributor.author | ISMAIL, AZIAH | |
| dc.date.accessioned | 2016-07-20T02:37:40Z | |
| dc.date.available | 2016-07-20T02:37:40Z | |
| dc.date.issued | 2009-01 | |
| dc.description.abstract | Typhoid fever caused by Salmonella Typhi remains a public health problem III underdeveloped and developing countries. Conventional culture method and biochemical tests to identify S Typhi lacked sensitivity, are laborious and produced results within 2 to 7 days. An alternative DNA test which is rapid, sensitive and userfriendly needs to be developed. Towards the development of such test, the gene encoding for the specific 50 kDa outer membrane protein of S Typhi (ST50) was used and primers were designed to amplify this DNA product with a size of 1238 bp. A PCR assay using such primers was evaluated against DNA extracted from pure cultures of 114 S Typhi and 66 non-S Typhi isolates. The assay showed sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of 100%. Re-evaluation of the PCR assay was performed with DNA of 25 S Typhi and 25 non-S Typhi isolates, with incorporation of an internal amplification control (lAC). The results again showed sensitivity, specificity, positive predictive value and negative predictive value of 100% in detecting S Typhi. From the above results, it was concluded that ST50 gene can be successfully utilised as a target for the detection of S Typhi by means of PCR. | en_US |
| dc.identifier.uri | http://hdl.handle.net/123456789/2292 | |
| dc.subject | Innovative Approaches Towards Development And Utilization | en_US |
| dc.subject | Of Dna Diagnostics For Salmonella Typhi | en_US |
| dc.title | Innovative Approaches Towards Development And Utilization Of Dna Diagnostics For Salmonella Typhi | en_US |
| dc.type | Thesis | en_US |