The effect of glucose concentration on the co-factor recycling in a non-growing whole-cell saccharomyces cerevisiae-mediated ketoisophorone biotransformation
| dc.contributor.author | Nanthini A/P Umapathy | |
| dc.date.accessioned | 2021-05-05T02:36:07Z | |
| dc.date.available | 2021-05-05T02:36:07Z | |
| dc.date.issued | 2017-05 | |
| dc.description.abstract | The aim of this study is to investigate the effect of different glucose concentrations on the recycling of co-factor in a non-growing whole-cell Saccharomyces cerevisiae. Besides that, this study also investigates the effect of different glucose concentrations on the biotransformation of 2,6,6-trimethylcyclohex-2-ene-1,4-dione also known as ketoisophorone in a non-growing whole-cell S. cerevisiae. The liquid phase biotransformation was carried out in a shake-flask culture. The conditions of biotransformation are 37 °C, 150 rpm, 5 g/L S. cerevisiae, 0.2 g/L ketoisophorone and varied concentration of glucose (5 g/L, 10g/L, 15 g/L). It was found that level of co factors were shown at different glucose concentrations. This indicates that co-factor recycling process exist in this reaction.15 g/L of glucose showed the highest value of absorbance which is 0.4100. 5.018 × 1022 number of glucose molecules are present in 15 g/L glucose. The optimum concentration of glucose for the formation of actinol is 15 g/L. 15 g/L of glucose showed a maximum of 12 mole % of actinol formed. Levodione was the only intermediate formed during the biotransformation. Ketone reduction did not occur due to the inhibition of alcohol dehydrogenase caused by high concentration of NAD+ . Besides, ketone reduction has a slower reaction rate as compared to the reduction of carbon-carbon double bond by enoate reductase. | en_US |
| dc.identifier.uri | http://hdl.handle.net/123456789/13260 | |
| dc.language.iso | en | en_US |
| dc.title | The effect of glucose concentration on the co-factor recycling in a non-growing whole-cell saccharomyces cerevisiae-mediated ketoisophorone biotransformation | en_US |
| dc.type | Other | en_US |
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