Analysis of immunological responses of murine macrophages to hydroxyapatite

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dc.contributor.authorKannan Gopinath, Vellore
dc.date.accessioned2015-06-25T06:36:52Z
dc.date.available2015-06-25T06:36:52Z
dc.date.issued2006
dc.description.abstractThe aim of the present study was to analyze the immunological response of hydroxyapatite (HA) to RAW264.7 cells by determining HA-induced phagocytosis. Immunological parameters included in this study were the role of polymerization of actin and microtubule, protein kinase-C (PKC), nitric oxide (NO), interleukin-1~ (ll-1~) and tumor necrosis factor-a (TNF-a). The results showed that HA were phagocytosed by murine macrophage cell line (RAW264.7 cells) at different incubation time. Increased PI of HA-treated cells were paralleled with increased period of incubation. TEM (Transmission electron microscopy) analysis showed that HA particles were engulfed by the cells and located within cell vacuoles. Cytochalasin B or/and colchicine significantly inhibited phagocytic activity of the cells to both HA particles and latex bead in a dose-dependent fashion. Stimulated cells produced PKC enzyme right at the early stage of phagocytosis at 7 minutes. Pre-treated cells with Bisindolylmaleimide ingested fewer particles in a dose dependent fashion. NO production was less by HA stimulated cells than by latex bead-stimulated cells. Inducible nitric oxide synthase (iNOS) expression in both latex bead- and HAstimulated cells was observed at 7. 15, 30 and 60 minutes of incubation time. L-arginine enhanced but L-NIL inhibited both phagocytosis and NO production by HA-stimulated cells. HA stimulated the cells to release both IL-1 f3 and TNF-a in a timedependent fashion. In the presence of anti-murine IL-1 f3 and TNF-a, HA-induced phagocytic activity by RAW264.7 cells was significantly reduced. Therefore, the results of the present study suggest that HA particles may induce phagocytic activity of murine macrophages (RAW264.7 cells) in an actin and microtubule polymerization dependent mechanism, which may be mediated through PKC enzyme. NO may play a crucial role in HA-induced phagocytosis by RAW264.7 cells which may also depend on IL-1 ~and TNF-a.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/813
dc.language.isoenen_US
dc.subjectImmunological responsesen_US
dc.subjectHydroxyapatiteen_US
dc.titleAnalysis of immunological responses of murine macrophages to hydroxyapatiteen_US
dc.typeThesisen_US
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