Exploring the mechanism for in vitro production of anthocyanins from a rare wild orchid Anoectochilus spp.
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Date
2016
Authors
Subramaniam, Sreeramanan
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Abstract
Anoectochilus sp. dan Ludisia discolor, orkid permata liar daratan, tumbuh di kawasan hutan yang teduh. Orkid tersebut adalah komponen perubatan tradisional di Asia. Mikropropagasi adalah penting untuk memelihara dan membiak tumbuhan yang diingini. Dalam kajian ini, protocol-protokol pensterilan permukaan dan mikropropagasi berjaya dibangunkan untuk Anoectochilus sp. Dan Ludisia discolor melalui penggunaan OA% (w/v) HgCh. Kadar pencemaran paling rendah (7.7%) dan kadar tertinggi kelangsungan hidup dan pertumbuhan diperoleh pada 63.1% dan 22.5% masing-masing untuk Ludisia discolor.
Anoectochilus sp. and Ludisia discolor, the terrestrial wild jewel orchids, grow in shaded forest areas. The orchids are components of traditional medicine in Asia. Micropropagation is integral for conserving and multiplying desired plants. In this study, surface sterilisation and micropropagation protocols were successfully developed for Anoectochilus sp. and Ludisia discolor through the use of 0.4% (w/v) HgCh. The lowest contamination rate (7.7%) and the highest survival and growth rates were obtained at 63.1 % and 22.5% respectively for Ludisia discolor.
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Keywords
Anoectochilus , Mikropropagasi , orkid permata , pensterilan