Fermentation And Purification Of Lipase By Burkholderia Metallica Using Column Reactor

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dc.contributor.authorMohamad Redza, Nur Ezzreen Zafhrina
dc.date.accessioned2020-08-25T07:41:45Z
dc.date.available2020-08-25T07:41:45Z
dc.date.issued2020-06
dc.descriptionLatihan Ilmiahen_US
dc.description.abstractIn this work, fermentation was conducted for the feedstock and purification of lipase by Burkholderia metallica using a column reactor. The targeted parameter for this research such as salt stability, types and concentrations of polyethylene glycol(PEG) and salt, pH of extraction solution and flowrate ml/s to see the performance of extractive purification system. Aqueous impregnated resin system(AIRS) is applied using porous glass beads grafted with polyethylene glycol (PEG). An evaluation studies on AIRS were performed with various molecular weight of polyethylene glycol (PEG) (PEG 2,000, PEG 4,000, PEG 6,000) and different type of salts (sodium citrate, potassium citrate and sodium acetate) as phase component. Liquid extractant of PEG was immobilized on the inert support (porous glass beads) and correspond to the aqueous state phase to surmount the drawback of aqueous two phase extraction (ATPE). The technique combines liquid-liquid extraction with ease if operation of column chromatography.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/9904
dc.subjectTechnologyen_US
dc.subjectIndustrien_US
dc.subjectFermentationen_US
dc.subjectBurkholderia Metallicaen_US
dc.titleFermentation And Purification Of Lipase By Burkholderia Metallica Using Column Reactoren_US
dc.typeOtheren_US
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