Microsatellite Instability And Loss Of Heterozygosity In Oral Squamous Cell Carcinoma In A Malaysian Population
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Date
2012-05
Authors
Mat Jusoh, Julia Ashaliza
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Publisher
Universiti Sains Malaysia
Abstract
Loss of heterozygosity (LOH) and microsatellite instability (MSI) have been documented as important events of oral squamous cell carcinoma (OSCC). Five microsatellite markers D3S192, D3S966, D3S647, D3S1228 and D3S659 were selected on chromosome 3p because of high frequency of alterations reported in head and neck squamous cell carcinoma and the involvement of VHL (von Hippel Lindau) at 3p25-26 and the FHIT (fragile histidine triad) at 3p14.2 genes proven in many tumor types. A total of 50 archival tissue samples of OSCC and corresponding normal samples were analyzed for LOH and MSI status. The overall LOH for the markers selected on 3p was 56 out of 189 informative cases (29.6%). The most frequent LOH was identified at the marker D3S966 which is 18/42 (42.8%) of informative cases suggesting the presence of putative of TSGs in this loci. In this study, we found high MSI in D3S966 which is 28.6% of informative cases; this reveals the possibility of mutation of mismatch repair genes in this region. Frequent microsatellite alteration (MA) was observed in D3S966 (71.4%), D3S1228 (56.7%) and D3S192 (41.0%). There is no significant association between LOH with gender, tumour stage and differentiation grades. However, there is a significant association between tumour stage and differentiation grades with MSI status in OSCC in Malaysian population with p values of 0.002 and 0.035. There is also a significant association between MA and differentiation grades with a p value of 0.041.
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Keywords
Microsatellite instability and loss of heterozygosity , oral squamous cell carcinoma in a Malaysian population