Immobilization of lipase from candida rugosa using alginate bead
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Date
2018-06
Authors
Nursyarinie Nabihah Azeman
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Abstract
Applications of enzyme in industry are becoming more relevant these days.
In an effort to increase the application of enzyme, several techniques have been
introduced in the research. In the present work, lipase from Candida rugosa was
immobilized on alginate beads for possible applications. The enzyme was entrapped
by drop-wise addition of an aqueous mixture of sodium alginate and the biocatalyst
to the hardening solution of calcium ion. Effect of alginate concentration, lipase
concentration, temperature at optimum conditions and reusability of the enzyme were
investigated. An increased in alginate concentration give higher unit activity of the
enzyme. This techniques provided gives higher immobilization yield (%) and
activity yield at 99.17% and 2.365 IU/mg respectively. Under optimal conditions
generate by ANOVA analysis using Design of Experiment (DoE) software ( 0.03
g/ml of lipase concentration and 2% of alginate concentration), the lipase beads
retained enzyme activity equivalent to 70.28%. The optimum temperature suitable
for immobilized enzyme also studied. Enzyme activity increases when the
temperature was increased to 37'C, then decreases at higher temperature. The
optimum temperature was determined at 37'C with unit activity of 0.331 IU/mg. At
temperature 55'C, the activity declined to 0.037 IU/mg which suggested due to
denaturation of lipase structure.