Evaluation Of The Reproductive And Developmental Toxicities Of The Aqueous Extract Of Labisia Pumila Var. Alata In Female Rats
| dc.contributor.author | Mohd Puad @ Mohd Fuad, Wan Ezumi | |
| dc.date.accessioned | 2016-09-07T01:13:24Z | |
| dc.date.available | 2016-09-07T01:13:24Z | |
| dc.date.issued | 2009-10 | |
| dc.description.abstract | Labisia pumi/a var, a/ala (LPA) or Kacip Fatimah has been listed by the government of Malaysia as one of the priority species for a multi-disciplinary scientific study towards the development of scientific, evidence-based herbal medicine. The present study described in this thesis is part of the preclinical assessment of LPA to evaluate its safety profile. The aims of this study were to evaluate the potential effects of aqueous extract of LPA on reproductive and developmental toxicities in Sprague Dawley rats. A preliminary study was conducted using unstandardised extract of LPA at 2, 20, 200 and 400 mg/kg/day. Commercially prepared standard aqueous extract of LPA, registered as Biolabisia® at the doses of 2,20, 200,400, 1000 mg/kg/day or distilled water (control) were administered by gavaging to animals during the main studies. The Teratogenic study was conducted by treating pregnant animals with Biolabisia® during the period of organogenesis. Treatments of animals began from the time prior to mating until lactational periods of seven days in the Reproductive toxicity study and from before mating up to day 15 of pregnancy in the Fertility study. Virgin rats were treated with Biolabisia® for duration of three weeks in the Female toxicity study. A substantial number of reproductive and developmental parameters were assessed throughout this study. All numerical data were analysed using SPSS version 12.0.1. Results obtained indicated that the administration of Biolabisia® at all dose levels did not cause mortality nor show noticeably any treatment-related signs of toxicity on the physical appearance and behaviour of all the rats studied. There was a consistent trend with body weight changes of females in all the experiments performed but they showed no significant differences except for the significant reduction in the actual and corrected maternal body weights of Biolabisia® -treated animals in the Fertility study. Treatment with Biolabisia® did not result in teratogenicity in rats since no foetus exhibited external congenital malformations. The total number of corpora lutea, number of implantation sites, percentages of preimplantation loss and post-implantation death, number of resorptions, number of live foetuses, foetal body weight and foetal sex ratio were comparable among all experimental groups. The length of oestrous cycles and reproductive organs weights of rats were statistically unaffected by Biolabisia®. However, high number of animals with irregular cycles, presence of ovarian follicular cysts and a trend of increased serum progesterone and free testosterone levels that were observed in the Biolabisia® 1000 mg/kg/day dose group do raised some concern. Taking all the cumulative data together, the current findings suggest that oral treatment of Biolabisia® of up to 1000 mg/kg/day in female rats is not associated with significant deleterious effects. However, the body weight and oestrous cycle of the females may be affected if higher doses of Biolabisia® are administered for a longer duration. Studies involving non-rodent animal species need to be performed to complete the safety profile of this herb. | en_US |
| dc.identifier.uri | http://hdl.handle.net/123456789/2456 | |
| dc.subject | Medical Sciences | en_US |
| dc.subject | The Reproductive | en_US |
| dc.title | Evaluation Of The Reproductive And Developmental Toxicities Of The Aqueous Extract Of Labisia Pumila Var. Alata In Female Rats | en_US |
| dc.type | Thesis | en_US |