Analysis Of Phytochemical Compounds, Antioxidant And Antiproliferative Activity Of Aqueous Extracts Of Capsicum Fruits
| dc.contributor.author | ABDUL RAHIM, ROHANIZAH | |
| dc.date.accessioned | 2016-05-17T01:23:19Z | |
| dc.date.available | 2016-05-17T01:23:19Z | |
| dc.date.issued | 2015-10 | |
| dc.description.abstract | Pepper is one of the most popular vegetables taken daily. There is a lack of study and evidence on the effects of pepper extracts against cancer cell proliferation that relates to their phytochemical and antioxidant activities on Malaysia cultivar. The aims of this study are to analyze the phytochemical in pepper extracts using GCMS and HPLC system, to determine the antioxidant activity of pepper extracts using DPPH assay and to determine the antiproliferative activity of pepper extracts using MTS assay in Malaysia cultivar. Results showed that the phytochemical, antioxidant and antiproliferative activities differ between Capsicum frutescens (chilli padi), Capsicum annuum (chilli pepper) and Capsicum annuum (bell pepper) extracts in Malaysia cultivar. The content of phenolic compounds (124.77 ± 1.70 mg/g GA equivalent) and antioxidant activity (520.68 mg/g ± 14.57 mg/g BHA equivalent) were highest in the bell pepper extract. The content of flavonoid (55.12 ± 0.27 mg/g Quercitin equivalent), capsaicin (1.09 ± 0.03 mg/g) and dihydrocapsaicin (0.63 ± 0.07 mg/g) compounds and antiproliferative activities were highest in the chilli padi extract. No relationship was found between DPPH antioxidant and antiproliferative activities of pepper extracts in Malaysia cultivar. Pepper extracts were not toxic to the Jurkat cells. At an equal concentration of 1 mg/mL, chilli padi extract has a higher inhibitory activity to Jurkat cells, which was 63 % than chilli pepper extract which was 30 %. Meanwhile bell pepper extract did not show any inhibitory activity against Jurkat cells. These results suggest the importance of volatile organic and phenolic compounds for antioxidant activity of bell pepper extract and the importance of flavanoid and capsaicinoid compounds for antiproliferative activity of chilli padi extract in Malaysia cultivar. The results on the antioxidant and antiproliferative activities from Malaysia cultivar also showed different activities from previous studies in other cultivar. These findings suggested that different species, varieties and cultivar have contributed to the different phytochemicals present in pepper. The different phytochemicals present contributed to the different antioxidant and antiproliferative activities of pepper extracts. The results indicate that chilli padi from Malaysia cultivar might be give more beneficial effects to human as a potential source of bioactive compounds for natural antioxidant and chemopreventive agents. | en_US |
| dc.identifier.uri | http://hdl.handle.net/123456789/2040 | |
| dc.subject | Analysis Of Phytochemical Compounds, Antioxidant And Antiproliferative | en_US |
| dc.subject | Activity Of Aqueous Extracts Of Capsicum Fruits | en_US |
| dc.title | Analysis Of Phytochemical Compounds, Antioxidant And Antiproliferative Activity Of Aqueous Extracts Of Capsicum Fruits | en_US |
| dc.type | Thesis | en_US |