Drug release study of pla microspheres loaded with gentamicin
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Date
2017-06
Authors
Nur Afiqah Binti Hamat
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Abstract
Poly(lactic acid) (PLA) microspheres were fabricated through emulsion and
solvent evaporation (ESE) technique. PLA loaded with different concentration of
gentamicin were immersed in 0.5M NaOH solutions for 48 hours. The concentration of
gentamicin were used are 0.125 %, 0.25 % and 0.375 %. Treated PLA microspheres
were investigated using several characterization methods including Fourier Transform
InfraRed (FTIR), Scanning Electron Microscopy (SEM), Particle size analysis, Contact
Angle and Ultraviolet (UV) visible spectroscopy. The peak of gentamicin was determined
and confirmed by FTIR. The treated PLA microspheres has becomes rough and porous
surface after surface hydrolysis treatment. SEM proved the surface morphology
changes of surface modified PLA microspheres. The value of contact angle was less
than 90° after surface hydrolysis treatment and confirmed the improved hydrophilicity
properties of surface modified PLA microspheres. The percentage of encapsulation
efficiency (% EE) of gentamicin within PLA microspheres was evaluated by using UV vis spectroscopy. The results recorded that higher concentration of gentamicin, which is
0.375 % gentamicin has the higher encapsulation efficiency around 15.22 %. The drug
release of gentamicin from PLA microspheres showed the burst-effect manner within
first 8 hours. After that, the drug release rate was slow and sustained release for the
remainder days monitoring period.