Cloning and expression of desaturase and elongase genes at various stages of oogenesis in female zebrafish Danio rerio
| dc.contributor.author | Alexender Chong, Shu Chien | |
| dc.date.accessioned | 2017-08-02T08:05:19Z | |
| dc.date.available | 2017-08-02T08:05:19Z | |
| dc.date.issued | 2008 | |
| dc.description.abstract | Although unsaturated fatty acids such as eicosapentaenoic acid (EPA, C20:5n-3), docosahexaenoic acid (DHA, C22:6n-3) and arachidonic acid (ARA, C20:4n-6), collectively known as the highly unsaturated fatty acids (HUFA), play pivotal roles in vertebrate reproduction, very little is known about their synthesis in the ovary. The zebrafish display capability to synthesize all three HUFA via pathways involving desaturation and elongation of two precursors, the linoleic acid (LA, C18:2n-6) and linolenic acid (LNA, C18:3n-3). As a prerequisite to gain full understanding on the importance and regulation of ovarian HUFA synthesis, we described here the mRNA expression pattern of two enzymes; desaturase (fadsd6) and elongase (elovl5) in different zebrafish ovarian follicle stages. mRNA levels of fadsd6 and elovlS in different ovarian follicle stages were determined by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) assays. For analysis of the ovarian follicular fatty acid composition; gas chromatography was used. Our results have shown that both genes displayed significant upregulation in expression during the oocyte maturation stage. Fatty acid composition analysis of different ovarian follicle stages also showed that ARA and DHA levels are significantly elevated in maturation stage. Collectively, our findings strongly suggest the existence of a HUFA synthesis system in zebrafish ovary, responsible for the supply of HUFAs for maturation and possibly ovulation processes. The versatility and many advantages of zebrafish as model system for reproduction will provide a useful platform to further elucidate the regulatory and mechanism aspects of ovarian HUFA synthesis. | en_US |
| dc.identifier.uri | http://hdl.handle.net/123456789/4301 | |
| dc.title | Cloning and expression of desaturase and elongase genes at various stages of oogenesis in female zebrafish Danio rerio | en_US |
| dc.type | Technical Report | en_US |