This study aimed to investigate the diagnostic and prognostic relevance of miR-484 in CC using integrated bioinformatics, and to functionally validate its role in regulating apoptosis through both in vitro and in vivo models. Particular focus was placed on the interaction between miR-484 and Polyalthia longifolia methanol extract (PLME), a natural compound previously shown to induce apoptosis in HeLa cells via miR-484 downregulation.