Institut Penyelidikan Perubatan Molekul - Tesis

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    Immunogenicity Evaluation Of A133 And Ss-Ir: Development Of Vaccine Candidates Against Strongyloides Stercoralis
    (2024-02)
    Wong, Matthew Tze Jian
    Strongyloidiasis, caused by the nematode Strongyloides stercoralis, remains a threat to global public health. To date, a vaccine against strongyloidiasis remains unavailable and there was no significant development in this area after discovering the potential of recombinant protein Ss-IR as a vaccine candidate in 2011. In light of recent developments in the diagnosis of strongyloidiasis, A133 emerged in 2021 as an excellent diagnostic antigen, prompting curiosity for its potential as a vaccine candidate. Therefore, this study aimed to evaluate the efficacy of recombinant protein A133 in comparison to Ss-IR, as a potential vaccine candidate against S. stercoralis by investigating humoral and cellular immune responses in immunised mice.
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    Influence Of Coaching Communication By Middle-Leaderstowards Teacher's Performance:Teaching Self-Efficacy As Mediator And Trust Towards Coach As Moderator
    (2024-02)
    Nazrin, Marina
    The study generally aims to identify practices executed by secondary school teachers with the influence of coaching communication by middle leaders towards their performance. Specifically, the study also aims to identify the role of trust towards coach as the potential moderator variable between coaching communication and teaching self-efficacy while the role of teaching self-efficacy in this study is the mediator towards coaching communication and teachers’ performance.
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    Generation And Characterization Of Scfv Antibody Fragment Targeting Japanese Encephalitis Virus Nsl From A Semi-Synthetic Phage Display Library
    (2019-08)
    Chong, Hui Ying
    Japanese encephalitis (JE) which is caused by Japanese Encephalitis Virus (JEV) is a flavivirus disease and remains to be the major form of viral encephalitis in Asia. JEV infection causes meningitis, encephalitis and in some cases, permanent damage to the brain or even death. As JEV causes severe pathophysiological conditions, we set to generate antiJEV NS 1 antibodies for future immunodiagnostic development.
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    Development Of Dengue Immunodiagnostic Assay By Targeting Denv Type 2 Ns1 Using Shark Single Domain Vnar Antibody
    (2024-07)
    Kok, Boon Hui
    Denv infection diagnosis requires early and rapid detection which is accurate and specific to provide effective treatment for the patients. However, problems such as limitations of rapid diagnostic test, antibody instability, cross-reaction among denv serotypes and other flaviviruses are causing the dengue diagnosis to be challenging. Alternatively, vnar sdab derived from shark ignar has proven some promising characteristics such as improved stability, thermostability and ability to bind on cavities or clefts hidden on targeted surface. These could possibly overcome the limitations encountered while using conventional antibodies. In this study, a potential binder namely anti-ns1 vnar-z8 was isolated from semi-synthetic shark vnar library and successfully expressed as soluble protein using e. Coli expression system. This soluble recombinant anti-ns1 vnar-z8 shown improved specificity (1.70 ± 0.11) towards denv type 2 ns1 antigen as compared to commercial anti-ns1 mab (1.17 ± 0.07). Besides, both antibodies were sensitive within the detection range of 0.03 μg/ml to 60 μg/ml. The good thermostability characteristic of anti-ns1 vnar-z8 was also proven in this study with retainment of binding affinity towards ns1 antigen after thermal treatment at various temperature (25°c to 60°c) for up to 1 week. On the other hand, the early lfa prototype was successfully developed using aunps conjugated anti-ns1 vnar-z8 protein. Throughout the prototype stability characterization,
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    Characterization And In Vitro Study Of Protoporphyrin Ix (Haem) Aptamer In Reversing Drug-Resistant Malaria
    (2024-07)
    Abdulwahab, Aliyu
    The global health challenge of malaria, compounded by drug resistance, necessitates innovative approaches for effective treatment. Aptamer technology is a promising tool towards combatting drug-resistant malaria especially chloroquine (cq) resistance. Preliminary study on protoporphyrin ix (haem) dna-aptamers (oka_24 and oka_26 ) demonstrated an anti-malarial property but lack the ability to internalise into the parasite-infected red blood cell (rbc). This research investigates the potential of cholesterol-tri ethylene glycol (col-teg) modified haem aptamers in addressing drug-resistant malaria. The research employs a multifaceted approach, including in silico techniques for predicting aptamer structures and molecular docking studies to assess binding behaviour. Additionally, reverse-phase high-performance liquid-chromatography (rp-hplc) was utilized to evaluate serum stability, while uv-absorption spectral titration and square wave voltammetry (swv) provided insights into the specificity and affinity of modified aptamers for haem. Cellular internalization assays, conducted using fluorescence-microscopy and flow cytometry, determine the efficiency of col-teg-modified aptamers in entering red blood cells. The study also examines the antimalarial activity of modified aptamers against cq-sensitive (3d7-strain) and cq-resistant (w2-strain) plasmodium falciparum. Docking analysis reveals that the transformation of oka_26 to col-teg-oka_26 does not alter binding behaviour,