Institut Penyelidikan Perubatan Molekul - Tesis
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- PublicationDevelopment Of A Chronological Life Span Assay For Ageing Studies In Saccharotnyces Cerevisiae(2019-06)Ong, Tee GeeThe Saccharomyces cerevisiae. chronological life span (CLS) is the length of time that yeast cells survive in a non-dividing state. In the laboratory, CLS is typically measured by its optical density at the post-diauxic phase that begins approximately two days after initial inoculation. In this study, the development of a CLS assay on a 96-well microplate with different yeast strains and growth parameters (media and time) were carried out. After the evaluation of four different strains, yeast strain BY25929 was found to be the most suitable in tenns of growth for the CLS assay. A total of 24 ageing-related proteins were selected for assessment in the CLS assay.
- PublicationTranscriptome Study Of Salmonella Enterica Subspecies Enterica Serovar Typhi Biofilm(2018-08)Jason, Chin Khee ChianTyphoid fever is an acute systemic infection of humans caused by Salmonella enterica subspecies enterica serovar Typhi (S Typhi). During infection the bacteria forms biofilm in the gallbladder to protect itself from the harsh environment of the host. Biofilm formation has been associated with increased resistance towards antibiotics and the host immune system, and promotes bacterial persistence. However, the mechanism of biofilm formation is unknown. High-throughput sequencing using the Illumina HiSeq 2500 platform was performed to study the transcriptome of S. Typhi planktonic, intermediate and mature biofilm cells to identify the genes involved in biofilm formation and to postulate the mechanism of action. Planktonic S.
- PublicationIsolation And Characterization Of Shark Vnar Antibody Against Malaria Pfhrp2 Protein From Semi-Synthetic Library(2019-08)Cheong, Wei ShienUjian diagnostik pantas (RDT) malaria adalah penguji imun berasaskan antibodi yang menpercepatkan diagnosis malaria. Walau bagaimanapun, reagen antibodi yang digunakan secara meluas untuk RDT malaria tertakluk kepada degradasi pada suhu sekitar yang tinggi yang boleh memendekkan jangka hayat RDT malaria.
- PublicationIntensification Of Core Streptavidin Production Through High-Cell-Density Cultivation Of Recombinant Escherichia Coli And A Temperature-Based Refolding Method(2019-05)Chua, Leong HuatThis study describes the development of an intensified process that enables high level production of recombinant core streptavidin (core SAV), a non-glycosylated tetrameric protein utilised in a wide range of applications. Due to widespread utility and commercial importance of SAV, recombinant SAV production has been studied extensively. However, in most studies, recombinant SAV was expressed in a soluble form, resulting in in vivo sequestration of biotin. Biotin sequestration is detrimental to cell growth and results in low volumetric yield. To improve volumetric yield, in this study, core SAV was expressed as inclusion bodies (IBs) followed by induction at highcell- density. A pH-stat fed-batch feeding strategy was employed to cultivate host cells to high-cell-density, the effect of induction at different cell densities (OD 20, 60 and 100) on volumetric and specific yield were then studied. Highest volumetric yield of core SAV (1.55 g L"1) was obtained from induction at OD 100.
- PublicationIn Vitro And In Vivo Studies On The Complementary Effects Of Standardized Ethanolic Orthosiphon Stamineus Extract On Pancreatic Cancer Cells(2018-03)Salem Yehya, Ashwaq HamidPancreatic cancer is globally known as the fourth most lethal cancer in the world. Despite increasing understanding in tumor biology; the efficiency of treatment in pancreatic cancer remains challenging as patients demonstrate resistance to standard treatments. Although the first line agent, gemcitabine has produced clinical response to treat pancreatic cancer, the prognosis remains dismal.