Institut Penyelidikan Perubatan Molekul - Tesis

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Study on effect of surface roughness of no tie bar (ntb) lead frame to mitigate delamination in small outline integrated chip (soic) packaging
(2025-09-01)
Thanigesh, Thiyagu
The widespread application of SOIC as digital isolators in various domains has raised high interest by the industrial key players. Due to constant drive for faster data rates, better performance and high operation rate, SOIC digital isolators are commonly subjected to higher mechanical and thermal stress in long term application which leads to delamination issue. Delamination has resulted in functionality failure of the chip in end user applications. To mitigate delamination, correlation between no tie bar (NTB) lead frame and surface roughness was studied by examining the critical to quality (CTQ) criteria in wire bonding and molding process parameter. The research work investigated two types of samples which were roughened and non – roughened NTB lead frame. Chips were manufactured through packaging assembly process using both frame samples followed by reliability test assessment such as moisture sensitivity level (MSL), unbiased highly accelerated stress test (UHAST), temperature cycle and high temperature storage (HTS). Characterization study of frames was done with scanning electron microscopy (SEM), atomic force microscopy (AFM), energy dispersive X – ray (EDX), optical microscopy, scanning acoustic topography (SAT) and X – ray. The ball shear, wire pull, stitch pull output response for roughened frame was higher than in non – roughened frame in wire bonding process. Whereas in molding process output response, roughened frame displayed better control in horizontal mean offset unlike non - roughened frame which had better control in vertical mean offset. Wire sweep and mold void was comparable in both samples meeting requirements. In reliability test, chips using roughened NTB lead frame demonstrated greater reliability with no delamination compared to non – roughened frame chips at post MSL, UHAST, temperature cycle and HTS test.
Immunogenicity Evaluation Of A133 And Ss-Ir: Development Of Vaccine Candidates Against Strongyloides Stercoralis
(2024-02)
Wong, Matthew Tze Jian
Strongyloidiasis, caused by the nematode Strongyloides stercoralis, remains a threat to global public health. To date, a vaccine against strongyloidiasis remains unavailable and there was no significant development in this area after discovering the potential of recombinant protein Ss-IR as a vaccine candidate in 2011. In light of recent developments in the diagnosis of strongyloidiasis, A133 emerged in 2021 as an excellent diagnostic antigen, prompting curiosity for its potential as a vaccine candidate. Therefore, this study aimed to evaluate the efficacy of recombinant protein A133 in comparison to Ss-IR, as a potential vaccine candidate against S. stercoralis by investigating humoral and cellular immune responses in immunised mice.
Influence Of Coaching Communication By Middle-Leaderstowards Teacher's Performance:Teaching Self-Efficacy As Mediator And Trust Towards Coach As Moderator
(2024-02)
Nazrin, Marina
The study generally aims to identify practices executed by secondary school teachers with the influence of coaching communication by middle leaders towards their performance. Specifically, the study also aims to identify the role of trust towards coach as the potential moderator variable between coaching communication and teaching self-efficacy while the role of teaching self-efficacy in this study is the mediator towards coaching communication and teachers’ performance.
Generation And Characterization Of Scfv Antibody Fragment Targeting Japanese Encephalitis Virus Nsl From A Semi-Synthetic Phage Display Library
(2019-08)
Chong, Hui Ying
Japanese encephalitis (JE) which is caused by Japanese Encephalitis Virus (JEV) is a flavivirus disease and remains to be the major form of viral encephalitis in Asia. JEV infection causes meningitis, encephalitis and in some cases, permanent damage to the brain or even death. As JEV causes severe pathophysiological conditions, we set to generate antiJEV NS 1 antibodies for future immunodiagnostic development.
Development Of Dengue Immunodiagnostic Assay By Targeting Denv Type 2 Ns1 Using Shark Single Domain Vnar Antibody
(2024-07)
Kok, Boon Hui
Denv infection diagnosis requires early and rapid detection which is accurate and specific to provide effective treatment for the patients. However, problems such as limitations of rapid diagnostic test, antibody instability, cross-reaction among denv serotypes and other flaviviruses are causing the dengue diagnosis to be challenging. Alternatively, vnar sdab derived from shark ignar has proven some promising characteristics such as improved stability, thermostability and ability to bind on cavities or clefts hidden on targeted surface. These could possibly overcome the limitations encountered while using conventional antibodies. In this study, a potential binder namely anti-ns1 vnar-z8 was isolated from semi-synthetic shark vnar library and successfully expressed as soluble protein using e. Coli expression system. This soluble recombinant anti-ns1 vnar-z8 shown improved specificity (1.70 ± 0.11) towards denv type 2 ns1 antigen as compared to commercial anti-ns1 mab (1.17 ± 0.07). Besides, both antibodies were sensitive within the detection range of 0.03 μg/ml to 60 μg/ml. The good thermostability characteristic of anti-ns1 vnar-z8 was also proven in this study with retainment of binding affinity towards ns1 antigen after thermal treatment at various temperature (25°c to 60°c) for up to 1 week. On the other hand, the early lfa prototype was successfully developed using aunps conjugated anti-ns1 vnar-z8 protein. Throughout the prototype stability characterization,

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