Publication: In vitro evaluation of polyphenolic-rich fraction of cornsilk extract as anti-hyperglycaemic agent
| dc.contributor.author | Joanne, Koo | |
| dc.date.accessioned | 2026-01-19T06:43:30Z | |
| dc.date.available | 2026-01-19T06:43:30Z | |
| dc.date.issued | 2025-01 | |
| dc.description.abstract | Diabetes mellitus (DM) is a chronic metabolic disease that characterised by elevated blood glucose level due to insulin resistance or inadequate insulin production. The increased blood glucose concentration also known as hyperglycaemia which contributes to various diabetic complications such as diabetic cardiomyopathy and diabetic nephropathy. Metformin and acarbose are the well-known medical practical drugs used to treat and alleviate the diabetes-related complications. However, those antidiabetic drugs bring side effects to the diabetic patients during treatment. Hence, this study is aimed to investigate the polyphenolic-rich fraction (PRF) of cornsilk extract as a potential candidate in the study of type 2 diabetes mellitus (T2DM). The antiglycation effect and mechanism of PRF of cornsilk was evaluated using in vitro study. The cell viability and EC50 of PRF of cornsilk were determined using murine fibroblast (L929) cell line via MTT assay. The PRF of cornsilk showed statistically significantly protected and enhanced the L929 cell viability and proliferation under high glucose with p value of < 0.0001 and EC50 value of 3.84 x 10-12 mg/mL. Upon the MTT assay, the PRF of cornsilk was more effective than the metformin in protecting cells. Furthermore, the PRF of cornsilk depicted statistically significantly (IC50 = 8.38 mg/mL) inhibited the α-amylase enzyme using in vitro α-amylase inhibition assay. However, acarbose demonstrated more robust with a greater inhibition effect (IC50 = 6.95 mg/mL) than the PRF of cornsilk in the α-amylase inhibition assay. Lastly, the PRF of cornsilk showed statistically significantly reduced the advanced glycation end products (AGEs) level using BSA-glucose and -methylglyoxal antiglycation assays. The PRF of cornsilk showed stronger antiglycation activity than the metformin in these antiglycation assays. In short, the PRF of cornsilk is proved with its effective antiglycation property and showed to be a promising candidate to reduce diabetes-related complications by antiglycation pathway. However, more investigations have to carry out to gain further understanding on the antiglycation effect of the PRF of cornsilk | |
| dc.identifier.uri | https://erepo.usm.my/handle/123456789/23468 | |
| dc.language.iso | en | |
| dc.subject | - | |
| dc.title | In vitro evaluation of polyphenolic-rich fraction of cornsilk extract as anti-hyperglycaemic agent | |
| dc.type | Resource Types::text::thesis::bachelor thesis | |
| dspace.entity.type | Publication | |
| oairecerif.author.affiliation | Universiti Sains Malaysia |