Publication: Malaysian propolis extract alleviates macrovascular complication in diabetic rats: pharmacological potential of natural bee product
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Date
2018
Authors
Yeoh, Boon Seng
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Abstract
Malaysian propolis (MP) derived from stingless bee: (Heterotrigona itama) possesses antihyperglycemic effect in vivo and show antioxidant activity. The aim of the study was to observe
the histological changes in aorta of streptozotocin-induced diabetic rats following MP
administration. Thirty adult male Sprague Dawley rats were randomly assigned into five
groups (n=6/group) of normoglycemic control (A): diabetic (DM) control (8); DM with
metformin (C); DM with MP (D); DM with metformin and MP combination (E).
Intraperitoneal streptozotocin (60mg/kg body weight) was employed to induce diabetes
mellitus. Distilled water (I ml/day) as vehicle (control). met form in (300mg/kg/day) and MP
(300mg/kg/day) were administered orally for four weeks, immediately after successful diabetic
induction. The arch of aorta was harvested for histological study using Hematoxylin & Eosin
and Verhoeff-Van Gieson stains. Aorta cross sections were examined under light microscope
for changes in tunica intima media. Tissues deposition in tunica intima marks the outset of
accelerated atherosclerosis in diabetes mellitus. The mean (standard error) or intimal thickness
was found to be 2.83 (0.21), 4.52(1.00), 2.68 (0.39), 2.54 (0.36) and 2.11 (0.35) µm for group A, B, C, D and E respectively. This study found a significant increase ( 1.6 fold) in tunica intima
thickness in the DM group compared to the normoglycemic control (p<0.05 ). There was no
significant difference in tunica media thickness and number of elastic lamellae. ln conclusion,
we infer that supplementation with MP modifies early progression or diabetic macrovascular
complication and defers adverse sequalae or diabetes mellitus on aorta. with additional
mechanism beside its anti-hyperglycemic effect. warranting future research.
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Keywords
Malaysian propolis