Pusat Pengajian Sains Kesihatan - Monograf

Browse

Now showing 1 - 5 of 602

Cell surface phenotype of lipopolysaccharides-stimulated peripheral blood mononuclear cells-derived microparticles
(2016)
Noor, Nur Azira Mohd
Monocytic microparticles (mMP) can be defined as a heterogeneous population of small vesicles with approximate size 0.1 to 1 gm derived from peripheral blood monocytes. Notable elevated levels of circulating microparticles (MP) have been observed in various clinical states, and are significantly associated with disease severity. Previous studies suggest that MP express phosphatidylserine (PS) which contribute in inflammation process. Their release is enhanced by cell injury, cell activation, or apoptosis and can be triggered by lipopolysaccharides (LPS) stimulation. MP display phenotypic and cytosolic compositions that resemble their parental cells. However, study on mMP is limited. To date, the characterization of cell surface mMP is unclear. In this study, we intended to characterize mMP by measuring cell surface expression of mMP including CD 14, CD 16 and PS as well as comparing the level of mMP secretion between stimulated and unstimulated peripheral blood mononuclear cells (PBMC). PBMC were cultured in the presence or absence of LPS for 18 and 24 hours. Monocytic MP secretion was assessed in the supernatants using anti-human CD 14, anti-human CD 16 and Annexin-V. Meanwhile, stimulated PBMC was assessed in culture pellet using anti-human CD14, anti-human CD16, and anti-human CD1 lb. All analyses were performed using flow cytometry. Our experimental data showed that CD 14 and Annexin-V marker were clearly detected on mMP. In contrast, CD 16 marker was undetectable. We observed that mMP production was proportional to stimulation period. LPS-slimulated PBMC secreted higher level of mMP compared to unstimulated PBMC when cultured for 18 and 24 hours. Cell viability of stimulated PBMC remains unchanged. In addition, changes in mean fluorescense intensity 'AM1T of all markers in unstimulated PBMC was higher than stimulated PBMC. These finding suggest that Annexin-V in combination with CD 14 are the potential cell surface markers for mMP detection, while we confirmed that LPS down-regulate the expression of CD 14, CD 16 and CD 1 1 b on stimulated PBMC.
Determination of total fat and fatty acids composition in selected infant formula and follow up formula available in Malaysia.
(2016)
Nasarudin, Nur Atiqah
Infant formula and follow up formula is one of the main nutritional sources for infants and children. In this study, the total fat and fatty acid compositions of infant formula (n=6) and follow up formula (n=6) were analyzed. Fats were extracted using the Folch et al (1957) method. and fatty acid compositions (group and individual) were analyzed by gas chromatography. In infant formulas, saturated fatty acid (SFA) (1.442~20.715%), monounsaturated fatty acid (MUFA) (1.64o~lu.o84%) and polyunsaturated fatty acid (PUFA) (60.345-73.065%) were the major group of fatty acids detected. Follow up formula products contained 2.319-26.321% of total SFA content, whereas the content of MUFA and PUFA ranged from 0.712-17.774% and 44.258-91.406% respectively. In addition, the range of DHA in infant and follow up formula were 3.487-6.084% and 0.516-15.373% respectively. About 1.624- 4.456% of linoleic acid/linolenic acid was detected in the analyzed products (infant and follow up formula). A small amount of trans fatty acids (2.626-10.144%) were also detected in both infant anf follow up formula.
Impaired conciousness and quality of life among post-traumatic brain injury patients at Hospital Universiti Sains Malaysia.
(2016)
Razak, Nur Ariedayu Abdul
Traumatic brain injury is defined as a disruption in the normal function of the brain that can be caused by a bump, blow, or jolt to the head or a penetrating head injury. Impaired consciousness is the first change in a client who traumatic brain injury. Clients that had TBI may experience impaired consciousness as well as affect their quality of life. The objective of this study is to assess the association between impaired consciousness and quality of life among post-traumatic brain injury patients at Hospital Universiti Sains Malaysia. A cross-sectional survey using a set of self-administered questionnaire from the Quality of Life after Brain Injury Tool. This study was conducted by follow-up telephone survey. A total of 55 respondents were involved in this study. Data was analyzed using SPSS version 22.0 and Chi-Square test was used to analyze descriptive data. A total of 56.4% (n=31) reported that majority of the participants were from 18-39 years old. Majority of respondents were male patients ,45(81.8%). Most of the respondents had high severity of TBI 16 (100%) had low quality of life and mild severity of TBI 16 (76.2%) had high quality of life. This study revealed that there was a significant association between the severity of TBI and quality of life (^>=0.000), while there were no significant association between location of TBI and quality of life (/?=0.532) , there were not significant association between selected demographic data age (p=0.443) and gender (p=0.286) with quality of life among post TBI patients at Hospital USM. In conclusion, post-traumatic brain injury patients with severe injury experienced low quality of life.
Awareness on the impact of instant noodles consumptions among undergraduate Students In Health Campus, USM.
(2016)
Ameran, Nur Aqilah
Instant noodle is a highly processed food that lacks nutritive value. It had consumed by people nowadays due to reasonable price, tasty and easy to serve. Instant noodle consumption become rise and it also have the impact on health. The aim of this study was to determine the prevalence and awareness level on the impact of instant noodles consumption among undergraduate students in health campus, USM. A cross-sectional design was used in this study. Two hundred thirty two (232) students were recruited in this study by cluster random sampling of undergraduate students in health campus, USM from January until February 2016. A self-administered questionnaire was used to collect data. Data was analysed using the Statistical Package for the Social Sciences (SPSS) version 22.0 for windows and Stata software 13. Pearson Chi Square, Fisher’s Exact Test and One-way Anova analyses were conducted to explore the association between socio-demographic characteristic, health history and instant noodles consumption. A p value < 0.05 was considered statistically significant. One hundred twenty six respondents (54.3%) reported rare instant noodles consumption with moderate (mean=3.94) awareness level on the impact of instant noodle. 142 of respondent were categorized in normal body mass index (BMI), 20.3% of respondent underweight and 3.4% of respondent were obese. There was a significant association between gender and parental attention with prevalence of instant noodles consumption. Burning sensation on stomach (23.7%), bloating and irregular bowel movement (23.3%) and headache (18.1%) were the symptoms presented by respondents after consumed instant noodle. This study revealed the prevalence and awareness on the impact instant noodles consumption among students in health campus was moderate. The findings of the present study indicate a need for health education and promotion on impact of instant noodle to health and healthy nutrition to improve understanding about impact of instant noodles to health committee and public
Awareness towards osteoporosis among patient’s female relatives in orthopaedic wards at Hospital Universiti Sains Malaysia (Hospital USM)
(2016)
Zainudin, Nur Anisah
The level of awareness towards Osteoporosis is still low. The purpose for this crosssectional study is to determine the level of awareness towards Osteoporosis among patient’s female relatives in orthopaedic wards. This study also examined the differences between the level of awareness and the level of education as well as determined the relationship between age and the level of awareness. The subjects included 94 patient’s female relatives in the three orthopaedic wards at Hospital USM. They were selected using convenience sampling. Data were collected from December 2015 until February 2016 using Osteoporosis Prevention and Awareness Tool (OPAAT). The data were analysed using SPSS version 22.0 for mean, standard deviation, frequency, percentage, /2-value and r-value. Ethical approval was obtained from Research Ethics Committee (Human), USM. The overall awareness level towards Osteoporosis among patient’s female relatives was low (Af=14.95, SD=5A73). The majority of the female relatives got low awareness on the Osteoporosis in general (AY=4.87, 5D=2.324), consequences of Osteoporosis (Af=2.68, 50=1.255) and Osteoporosis prevention (A/=7.41, 50=3.303). The study found that there were significant differences between the level of education and the level of awareness among the female relatives with P = 0.017. Finally, there is positive but weak relationship between the age and the level of awareness among the female relatives with r = 0.027. Action should be taken to increase the awareness towards Osteoporosis among female relatives. In order to make sure the programme is effective, proper planning for health educational programme on Osteoporosis should be done. The expert health practitioner should be assigned so that the programme is beneficial.

Browse

Recent Submissions