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Proteomic And Biochemical Analysis Of Detoxification Enzyme In Musca Domestica L. (Diptera: Muscidae) And Its Response Upon Prolonged Exposure Of Insecticide

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dc.contributor.authorManoharan, Kalaavathi
dc.date.accessioned2025-03-24T03:25:28Z
dc.date.available2025-03-24T03:25:28Z
dc.date.issued2024-02
dc.description.abstractThis study provides the proteomic and biochemical information of the detoxification enzymes namely acetylcholinesterase (AChE), α-esterase (α-est), β- esterase (β-est), glutathione s-transferase (GST) and cytochrome P450 (Cyt P450) in susceptible (adult, larvae and pupae) and poultry strains (larvae) Musca domestica. Upon whole protein analysis, number of proteins discovered in larvae was highest (441), followed by adults (181) and pupae (115) which concluded specific proteins are necessary for the proper progression of each developmental phase. Meanwhile, the enzyme activities as well as the protein content of the susceptible strain were found to have significantly varied enzymatic activity across the developmental stages (p<0.05). Thirthy four distinct peptides of the targeted detoxification enzyme were discovered at various developmental stages of the susceptible strain. Functional analysis of the targeted peptides of the susceptible strains exposed that in addition to their functions in detoxification, the multifunctional enzymes were also found to be developmental-related proteins that responsible in various biological process, molecular functions and KEGG pathways to complete metamorphosis. Overall, it has been observed that at the larvae stage, the targeted developmental related detoxification peptides were more expressed and were the most enriched in terms of biological process, molecular function and KEGG
dc.identifier.urihttps://erepo.usm.my/handle/123456789/21352
dc.language.isoen
dc.subjectProteomic And Biochemical Analysis
dc.subjectDetoxification Enzyme
dc.subject(Diptera: Muscidae)
dc.subjectIts Response Upon Prolonged Exposure
dc.subjectResponse Upon Prolonged Exposure Of Insecticide
dc.subjectManoharan
dc.subjectKalaavathi
dc.subjectPusat Pengajian Sains Kajihayat
dc.titleProteomic And Biochemical Analysis Of Detoxification Enzyme In Musca Domestica L. (Diptera: Muscidae) And Its Response Upon Prolonged Exposure Of Insecticide
dc.typeResource Types::text::thesis::master thesis
dspace.entity.typePublication
oairecerif.author.affiliationUniversiti Sains Malaysia
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