Pusat Pengajian Sains Kajihayat - Tesis

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Endoparasitic Infections In Wild Rats And Their Distribution Using Geographic Information System (Gis) In Penang, Malaysia
(2025-02)
Perison, Putri Wulan Dari
Wild rat species are renowned for their capacity to act as reservoir hosts and carriers of diseases. Wild rats have effectively acclimated to various ecosystems and living conditions, even thriving in urban areas. Due to the close interaction between wild rats and the community, this study aims to determine the prevalence of endoparasite infections in wild rats from penang, malaysia as well as highlight zoonotic parasitic to understand more the severity of the parasitic infections that pose a risk to the public. A total of 150 wild rats were collected from commercial areas (n=86) and residential areas (n=64) around penang island and seberang perai. The sample was collected by collaborating with the pulau pinang city council (mbpp) and seberang perai city council (mbsp). In this study, four species of wild rats collected and identified as rattus rattus (58%), rattus norvegicus (15.33%), bandicota indica (24%) and bandicota bengalensis (2.67%). The endoparasites from gastrointenal, blood and fecal was examined through necropsy, blood smear, in – vitro cultivation and fecal floatation.
Application Of Light-Emitting Diodes On Eurycoma Longifolia Hairy Root Cultures: A Strategy For Enhanced Production Of Antiparasitic Compounds Targeting Blastocystis Sp.
(2025-03)
Abushattal, Mahmoud Ali Khalaf
Eurycoma longifolia (also known as tongkat ali) is a valuable medicinal plant that is frequently used in traditional medicine in malaysia. Its alkaloids and quassinoids reportedly confer antiparasitic effects. The effects of different light-emitting diode (led) spectral treatments on bioactive compounds synthesis, growth characteristics, total carbohydrate and protein content, antioxidant activity, and histological in e. Longifolia hairy root cultures (elhrcs) have not been explored, and the consequent antiparasitic effects on blastocystis sp., a common intestinal protozoan parasite, have not been determined. In this study, elhrcs were irradiated with white, blue, red, blue plus red (1:1) mint green light and dark as control in vitro. The variation in bioactive compound content was assessed by using high-performance liquid chromatography (hplc) were employed to quantify 9-hydroxycanthin-6-one, 9-methoxycanthin-6-one, and eurycomanone. In addition, the dichloromethane (dcm) crude extract was studied through the isolation, purification, and structural elucidation processes, resulting in the discovery of two known compounds, namely canthin-6-one and 9-methoxycanthin-6-one.
Biological Recovery Of Pha From Bacterial Cells Using Mealworms (Tenebrio molitor) And Black Soldier Fly Larvae (Hermetia illucens)
(2025-07)
Sivakumar, Lilyann Narishah
The production of polyhydroxyalkanoates (PHA) can be upscaled through fermentation and be chemically and biologically recovered from the bacterial cells to produce valuable plastic wares. PHA can be chemically recovered by using chloroform, but it is hazardous and costly. For biological recovery, the bacterial cells must be dry prior, to be a suitable insect feed through freeze-drying or drum-drying. These drying processes are not environmentally friendly, thus, hindering the commercial productions of PHA.
Genetic Diversity, Pathogenicity And Secondary Metabolites Of Fusarium Spp. Associated With Bakanae Disease Of Rice In Malaysia And Bangladesh
(2025-02)
Husna, Asmaul
Bakanae disease is an emerging rice disease that has become a major problem in asian rice growing countries, including bangladesh and malaysia. The disease can reduce rice production by up to 30%. Fusarium fujikuroi was known to cause bakanae disease, while only a few other fusarium species were associated with it. The current population of fusarium species may not be the same as previously reported due to changes in rice varieties, climate, taxonomic tool and population migrations. Therefore, the aim of the present study was to identify and characterise the fusarium species associated with bakanae disease of rice in malaysia and bangladesh based on morphological, biological, molecular, and physiological traits. In 2019-2022, a survey for bakanae-infected plants were conducted in different rice growing areas in malaysia and bangladesh. Accordingly, fusarium isolates were isolated from bakanae-infected plants. Twelve fusarium species were morphologically and biologically identified from 210 fusarium isolates. However, based on molecular identification using tef-1α and rpb2 genes, 17 fusarium species, namely, f. Fujikuroi, f. Verticillioides, f. Proliferatum, f. Andiyazi, f. Mangiferae, f. Longipes, f. Solani, f. Oxysporum, f. Graminearum, f. Commune, f. Hainanense, f. Bubalinum, f. Pernambucanum, f. Tanahbumbuense, f. Sulawesiense, f. Flagelliforme and f. Arcuatisporum were identified. Compared to bangladesh, a broad spectrum of fusarium species associated with bakanae disease of rice was found in malaysia.
N-Terminal Truncation Of Pha Synthase (Phacbp-M-Cpf4) As A Strategy For Improved Polyhydroxyalkanoate Properties By Cupriavidus Necator
(2025-01)
Neoh, Soon Zher
Polyhydroxyalkanoate (pha) is a type of bio-based and biodegradable polyester, synthesized by most bacteria and archaea under excess carbon and limited nutrient conditions. Among the types of pha, poly[(r)-3-hydroxybutyrate-co-(r)-3-hydroxyhexanoate] [p(3hb-co-3hhx)] is reported to have a close resemblance to the commercial polypropylene (pp) and low-density polyethylene (ldpe). Pha synthase (phac) is the most important enzyme in pha biosynthesis as it determines the properties of the pha produced. The structure of a phac consists of an n- and c-terminal. To date, there are not many productive phacs reported for the production of p(3hb-co-3hhx). Previous studies have shown that phac isolated from mangrove soil metagenome, phacbp-m-cpf4 is an efficient phac for p(3hb-co-3hhx) production and n-terminal influences substrate specificity and dimerization for the phac to function, granule morphology and molecular weight of pha produced. The full-length or nearly full-length structure of phacbp-m-cpf4 was successfully predicted using the alphafold software and psipred server. The n-terminal of phacbp-m-cpf4 was truncated based on its predicted structure giving rise to 4 n-terminal truncated mutants, phacbp-m-cpf4 g8, a27, t74, and d104 and cloned into cupriavidus necator phb−4.

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