Pusat Pengajian Sains Kajihayat - Tesis

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Effects Of Irrigation And Fertilizer Application On Water And Fertilizer Use And Soil Environment In Greenhouse Tomato
(2025-08)
Lyu, Xiaona
Efficient water and fertilizer management is crucial for optimizing greenhouse tomato (Solanum lycopersicon L.) production while mitigating environmental impacts such as soil degradation, nutrient loss, and pollution. This study aims to: (1) study the effects of water and fertilizer application patterns on growth, physiology, water consumption, yield, fruit quality, soil nutrients and microbial environment; (2) evaluate the response of the nutrient absorption of various parts of greenhouse tomato under different application of water and fertilizer; (3) propose the optimal combination of irrigation volume and fertilizer application suitable for efficient and high-quality tomato production in greenhouses.
Systematics And Biogeography Of The Genus Chitala (Osteoglossomorpha: Notopteridae) In Malaysia Using An Integrative Approach
(2025-09)
Ruzman, Luqman Hakim
The taxonomy of the freshwater featherback fish genus Chitala (Osteoglossomorpha: Notopteridae) remains unsettled because delimiting and naming Chitala species based solely on morphology led to different hypotheses. In this study, the number and identity of Chitala species present in Peninsular Malaysia were determined by employing an integrative taxonomic approach that combines morphological characters and two mitochondrial markers: the cytochrome c oxidase subunit I (COI) and cytochrome b genes.
Influence Of Aqualase And Grobiotic@- A On Growth, Digestive Enzyme Activities, Survival And Streptococcus Faecium Resistance In Rainbow Trout (Oncorhynchus Mykiss) Juveniles
(2022-08)
Azari, Abdolhamid
The present study was carried out to investigate the effects of supplementation with a probiotic, aqualase (thepax©) and a prebiotic, grobiotic,li)- a, respectively, on rainbow trout oncorhynchus mykiss, growth and health performance, two series of experiments were conducted to evaluate the use of these supplements in a commercial trout feed (chine, iran) against fish pathogenic bacteria, streptococcusfaecium and their effects on growth performance, haematological, immunological parameters and specific enzyme activities of rainbow trout, o. Mykiss. In both experiments, blood was sampled for haematological and immunological parameters after 4, 8 and 12 weeks, respectively of feeding. At the end of the trials, gastrointestinal tract of experimental fish were removed to assay for digestive enzymes activity and determination of the total bacterial count in the intestines. In the first experiment, five diets were prepared each comprising of a control (not supplemented); 1 and 2% g-a respectively and 0.1 and 0.2% aqualase. The diets were fed twice daily to triplicate groups of 300 o. Mykiss fingerlings (average 11.30 ± 0.21g) in concrete tanks for 12 weeks. The growth performance and survival rate were significantly higher in fish maintained on the both g-a and aq treatments compared with the control. However, final weight and survival fish fed g-a2% was the highest among all the treatments tested. Haematological parameters showed no significant difference between treatments, whereas immunological parameters consisting igm (56.10 ± 3.44 mg/ml) and lysozyme (12.27 ± 0.97 mg/ml) were found significantly higher in the fish fed with supplemented g-a2% between groups
Environmental Dna (Edna) As A Tool For Monitoring Native Belida Species (Chitala Lopis) And Introduced Belida Species (Chitala Ornata) In Malaysian Freshwater Ecosystem
(2025-09)
Adnan, Muhamad Hanif Iryani
The use of environmental dna (edna) methods offers significant advantages in terms of cost, time and labour for monitoring rare and introduced species. However, most of the edna studies to date have been conducted in temperate environments, while edna-based applications for monitoring tropical organisms, particularly freshwater fishes remain limited and lack standardised procedures. In this study, qpcr-based edna methods were used to detect the presence of two freshwater fish species from the genus chitala (family notopteridae), locally known as ‘ikan belida’. Chitala lopis is native to peninsular malaysia but is rare, whereas chitala ornata has been introduced in this region and may represent a threat to local ecosystems. A species-specific pair of primers incorporated with a probe-based assay was designed to detect c. Lopis, whereas a pair of primers designed by osathanunkul & minamoto (2020) coupled with sybr green-based assay was used for detecting c. Ornata. A series of validation steps were included to test the specificity and sensitivity of each method. Environmental water samples were collected from the two rivers where either species was reported, namely the perak river for c. Lopis and the perai river for c. Ornata. The possible impacts of several environmental factors on edna detection were evaluated by recording environmental parameters at each sampling site. Overall, edna of each species was successfully detected at their respective locations, but not at all sampling sites.
Integrative Taxonomy Of The Limnonectes Hascheanus-Limborgi (Anura: Dicroglossidae) Complex: Resolving Cryptic Species In Peninsular Malaysia
(2025-05)
Zou, Bei
Limnonectes hascheanus and l. Limborgi are two closely related species that are herein referred to as the limnonectes hascheanus–limborgi complex. In the past, l. Hascheanus was the only species reported to be present in peninsular malaysia. Matsui reported one of their examined specimens from janda baik as l. Limborgi. This study aimed to ascertain the status of both species in peninsular malaysia through an integrated approach, combining morphological, bioacoustic and molecular data. The results indicated that in peninsular malaysia there two different lineages of the l. Hascheanus–limborgi complex that can be separated genetically, by their male advertisement calls, as well as by external morphology. Both lineages differ by having a high genetic distance between them of 2.4–7.8% based on both mtdna 16s rrna and nudna tyrosinase genes. One lineage has a morphotype assignable to l. Hascheanus described from penang island and bears the following characteristics: slender body and light or dark–olive–brown color; toe webbing not reaching middle subarticular tubercle on the fourth toe; and the absence of enlarged odontoids in males. Populations from langkawi island; kedah, perlis, and some specimens from bukit larut, perak are genetically and bioacoustically similar to topotypic specimens of l. Hascheanus from penang island.

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