Pusat Pengajian Sains Kajihayat - Tesis

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In Vitro And In Silico Analysis Of Sphingobacterium Sp. Lipase Co-Expression With Lea K Peptide
(2025-10)
Ibrahim, Muhammad
A yellowish gram-negative bacterium called sphingobacterium sp. Is frequently distinguished by large quantities of sphingophospholipids in its lipid composition. Since few studies have been done on the sphingobacterium sp. Lipase, which has been the subject of limited research, this study aims to provide essential knowledge on the lipase activities of sphingobacterium sp. With lea-k as co-expression and in silico studies. To characterize ab3 lipase from sphingobacterium sp. That has been produce with lea k. To predict the 3-dimensional structure of sphingobacterium sp. Lipase and lea-k peptide using an in-silico approach. To investigate the interaction of ab3 lipase-lea k peptide complex that enhanced catalytic activity using in silico studies. Therefore, this study's results will be more helpful in providing a basic grasp of favourable application values for sphingobacterium sp. Lipase. Olive oil was used as the substrate in a colourimetric test technique to measure lipase activity. The lipase structure was predicted using the swiss-model website, and molecular docking was done using the autodock vina program to investigate molecular interactions. The optimum expression conditions were determined at 25ºc for 24 hours of incubation with 0.5mm iptg.
Effect Of Sugar Variants On The Aedes Mosquito Midgut Microbiome And Sugar-Feeding Behavior
(2025-07)
The Aedes mosquito species are known for spreading illnesses caused by dengue and Chikungunya virus. Control efforts have been strengthened by the employment of novel integrated vector management techniques such as alginate hydrogel beads in combination with toxic sugar bait. However, limited sources are available on the relation of toxic sugar bait with the attract and kill mechanism. Therefore, this study identified the fundamental properties of sugar variants that affect the behaviour of mosquitoes towards the sugar sources
Establishment Of Tissue Culture Planting Materials Of Mango (Mangifera Indica L.) Cv. Harumanis
(2025-03)
Lee, Zun Yip
Harumanis mango is one of the highest demand mango cultivars in malaysia due to its exceptional sweetness and fabulous fragrance. It is grown in a northern state of malaysia, perlis. However, the production of this mango cannot meet the market demand as there are several limitations in its propagation. The limited grafting activity and the limited number of seeds (only one harvest season per year) make vegetative and non-vegetative propagation of harumanis time and labour intensive. Micropropagation using tissue culture techniques is a reliable and effective alternative for mass in vitro propagation of harumanis at a consistent and faster rate, producing clones of the mother plants. Few studies have used nodal segments as starting material and the reported success rate is low. This is due to the two main problems, namely deep-seated contaminants and the recalcitrant nature of the plant. The objective of the present study is to develop an effective disinfection and shoot proliferation protocol for mangifera indica cv. Harumanis. A sterility rate of 87.5% was achieved by pretreating mother plants with 0.5 ml/l azoxystrobin two days before the experiment and surface sterilising nodal segments at the immature green leaf stage with 40% clorox® for 20 minutes, 0.5 g/l benomyl for 1 hour and 300 mg/l cefotaxime for 5 minutes. The transverse thin layer (ttcl) technique was applied to the nodal segments and cultures were maintained at wpm with 0.5 g/l benomyl and 300 mg/l cefotaxime.
In Vitro Multiplication And Plant Regeneration Of Red Banana (Musa Acuminata) Using Light-Emitting Diodes (Leds) System
(2025-03)
Bege, Jonathan
The red banana is a versatile plant with numerous nutritional benefits, agricultural potential and applications in food science and technology. Its special characteristics make it the subject of ongoing studies aimed at maximising its benefits and sustainability in a variety of industries. In the present study, the effects of different light spectra and plant growth regulators (pgrs) on the in vitro cultivation, physiological properties, genetic similarity and acclimatisation of musa acuminata cv. Red banana were investigated. The aim of the study was to improve shoot regeneration, growth indicators, root development and the successful transfer of plantlets to the field. In the first phase of the trial, thidiazuron (tdz) and 6-benzylaminopurine (bap) proved to be the most effective pgrs for promoting shoot formation, with tdz treatments resulting in the highest shoot regeneration rates. Tdz improved shoot multiplication but resulted in shorter shoot lengths than other pgrs. Indole-3-butyric acid (iba) was found to be most effective in root formation, with 1 μm significantly increasing root elongation. Naphthaleneacetic acid (naa) at 3 μm produced the largest average number of roots.
Endoparasitic Infections In Wild Rats And Their Distribution Using Geographic Information System (Gis) In Penang, Malaysia
(2025-02)
Perison, Putri Wulan Dari
Wild rat species are renowned for their capacity to act as reservoir hosts and carriers of diseases. Wild rats have effectively acclimated to various ecosystems and living conditions, even thriving in urban areas. Due to the close interaction between wild rats and the community, this study aims to determine the prevalence of endoparasite infections in wild rats from penang, malaysia as well as highlight zoonotic parasitic to understand more the severity of the parasitic infections that pose a risk to the public. A total of 150 wild rats were collected from commercial areas (n=86) and residential areas (n=64) around penang island and seberang perai. The sample was collected by collaborating with the pulau pinang city council (mbpp) and seberang perai city council (mbsp). In this study, four species of wild rats collected and identified as rattus rattus (58%), rattus norvegicus (15.33%), bandicota indica (24%) and bandicota bengalensis (2.67%). The endoparasites from gastrointenal, blood and fecal was examined through necropsy, blood smear, in – vitro cultivation and fecal floatation.

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