Publication: Characterisation and anti-asthmatic effects of polysaccharides from lignosus rhinocerotis in an acute airway inflammation model
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Date
2024-09
Authors
Rosdan, Bushra Solehah Mohd
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Abstract
murine model of asthma. Female BALB/c mice were sensitised with ovalbumin (OVA) and aluminium hydroxide at day 0 and day 14, followed by OVA challenges from day 21 to day 27. LRP (1, 10 and 100 mg/kg) and dexamethasone (DEX) (0.25 mg/kg) were administered intranasally following each challenge, with a normal group receiving only PBS treatment. Bronchoalveolar lavage fluid (BALF) was collected for cytokine analysis and differential blood count, serum for immunoglobulin E (IgE), lungs for histopathological, immunohistopathological and gene expression. The findings revealed that LRP exhibited a semi-crystalline structure primarily composed of glucose, with the predicted structural elements of →4)-α-D-Glcp-(1→ and →3)-β-D-Glcp-(1→. Cell viability studies demonstrated that LRP had no cytotoxic effects on Vero cell lines over 24-48 hours of treatment, with cell viability remaining above 80 %. LRP treatments significantly reduced Th2 cytokines production and IgE levels (p<0.05). Histological assessment revealed a decrease in inflammatory cell infiltration, mucus production, and TGF-β1 expression in the lungs of LRP treatment groups. Furthermore, gene expression analysis exhibited reduced COX-2, iNOS and Muc5ac expression in LRP treatment groups, with iNOS exhibiting a significant reduction. In conclusion, LRP has the potential to mitigate allergic inflammation in an OVA-challenged murine model, offering potential as an alternative approach for managing allergic asthma.