Publication: Pilot Study On Microbiota Profiling In Female Patient With Autoimmune Connective Tissue Disease From Hospital Putrajaya
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Date
2023-08
Authors
Wasdewa, Soonali
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Abstract
An autoimmune disease occur in human body when the immune system could not differentiate or recognise between foreign antigen and own cells and thus, attacks and destroys healthy cells and tissues in the own body. Autoimmune connective tissue disease (CTD) is a disease when the immune system affect the function and structure of connective tissues, such as joints, skin, eyes, gastrointestinal track, heart, and lungs mostly in female body. Gut micro biota play ab important role in human immune system by providing protection against pathibionts. However, imbalance of gnt microbiota occur in autoimmune patients resulting in curiosity whether the microbiome in the gut cause autoimmunity in the body or vice versa. This study therefore aimed to profile presence of gut microbiota in healthy versus autoimmune connective tissue disease (CTD) female patients. The stool samples and blood samples were collected from healthy and autoimmune patients and the DNA were extracted from stool samples using QIAamp PowerFecal Pro DNA kits. Furthermore, the extracted DNA was sequenced using 16S rRNA next generation sequencing (NGS). The produced sequences was aligned and analysed using Basic Local Alignment Search Tool (BLAST) and the identified microbes was profiled using MicrobiomeAnalyst webserver and produced heatrnaps of healthy control and autoimmune patients. The comparison of heatrnaps between the healthy control and patients showed both category have high abundance of Blautia argi (prevalence 1.0) which indicate the patients does not have imbalance of Blautia argi and also it does not cause the autoimmunity in the patients body. Furthermore, compared to healthy control, patients have high abundance of Bifidobacterium, and unclassified Lachnospiraceae genera (prevalence 1.0) which is abnormal and may contribute to autoimmunity. High abundance in Streptococcus genera also found in patients samples that have molecular mimicry ability in producing II homologous proteins similar to human proteins which may contribute to development of autoimmunity. The current study showed only the heatrnaps that indicate the imbalance of gut microbiome in the patients samples, but, it does not provide the understanding or evidence of causes of imbalance, causes of autoimmunity or influence of gut microbes in immune system. Thus, gut microbiota in CTD patients from Hospital Putrajaya and healthy control have been profiled and it serve as fundamental to establish the correlation between autoimmune and gut dysbiosis among Malaysian population.
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Medical , Pilot Study On Microbiota , Hospital Putrajaya , 2023 , Soonali