Institut Perubatan & Pergigian Termaju - Tesis

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    Optimization Of Immunoillstochemistry: Testing For Mlhl And Msh2 Proteins In Colon Tissue
    (2005-05)
    Muhsain, Siti Nur Fadzilah
    Microsatellite instability (MSI) accounts for the development of about 15% of all colorectal cancers. It is characterized by mutations in mismatch repair (MMR) genes that render cells unable to detect errors during DNA replication. Mutations in the MMR genes, MLHl and MSH2 are responsible for the majority of CRC with MSI. These MMR proteins can be detected by immunohistochemistry (lllC). IHC has proved sensitive and specific when compared to molecular analysis of MSI. Nevertheless, generally, there is no published literature as yet on studies of MSI in Malaysia, and specifically using lliC. Before any lliC staining procedure can be done, it has to be optimized. In this study, attempts were made to optimize rnc staining of MLHl and MSH2 proteins using polyclonal rabbit antibody by Biovision on formalin-fixed paraffm-embedded sections of normal colon, taken from resected margins of colectomy specimens. Either Vectastain Elite ABC Kit or DAKO En Vision Kit was used as secondary detection system. The staining procedure was done using different dilutions of the primary antibodies and incubated for different durations at different temperatures. Antigen retrieval procedures were introduced using citrate buffer pH 6 or Tris-EDTA buffer pH 9. The results showed that incubation of sections at room temperature using different dilutions of antibody failed to produce any nuclear staining. Staining with DAKO En Vision was still unsatisfactory, although background staining was reduced. Introduction of antigen retrieval procedure using microwave did not improve results. When incubated at 4°C, non-specific background staining was produced.
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    Determination Of Epstein-barr Virus In Gastrointestinal Lymphomas (With Special Reference To Non-hodgkin's Lymphoma Of Small Intestine): Study On Malaysian Patients.
    (2005-05)
    Yunos, Asmah Md
    Non-Hodgkin's lymphoma (NHL) is ranked twelfth among all cancers world-wide, in which it is more prevalent in males compared to females. The aim of the present study was to determine the presence and distribution of Epstein-Barr virus (EBV) in non-Hodgkin's lymphomas (NHL) of the gastrointestinal tract (GIT) tissue samples obtained from 25 Malaysian patients diagnosed clinically and histopathologically. The gastrointestinal lymphomas analysed in the present study was divided into NHL of the ileocecal (9 cases), NHL of the small intestine (7 cases), NHL of the stomach (5 cases), NHL of the abdomen (1 case), NHL of the wedge ulcer (1 case), NHL of rectum fotic (1 case) and finally NHL of the hemicolon (1 case). The presence of EBV in all of the above mentioned tissue samples, after formalin fixation and paraffin embedding was carried out by using conventional in situ hybridization technology. Two out of 25 cases of NHL of the GIT analysed in the present study, demonstrated positive signals for EBV/EBER. In the first positive case, EBV/EBER signals were located in transformed lymphocytes in the serosa layer of the small intestine (14.3%). In the second EBV positive case, EBV/EBER signals were located in diffuse B-cell lymphomas of the ileocecal (11.1 %). In conclusion, these finding demonstrate a rare association between EBV and lower gastrointestinal lymphomas (excluding stomach).
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    Development Of Multi-Loci Sequence-Based Genetic Diagnostic Testing Strategy For Severe Combined Immunodeficiency (Scid) Patients In Malaysia
    (2024-02)
    Habib Dzulkarnain, Syarifah Masyitah
    The central objective of this thesis was to establish a qPCR-based assay of SCID gene panel based on patients investigated for SCID that involving 9 genes: RAG1, RAG2, DCLRE1C, LIG4, ADA, IL2RG, JAK3, IL7Rα and PNP by using qPCR-based assay.
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    The Role Of Rarβ Gene On Cancer Stemness Capability In Non-Small Cell Lung Cancer Cell Line
    (2024-03)
    Abu Halim, Noor Hanis
    This study was conducted to understand the role of RARβ in CSCs maintenance by using lentivirusmediated shRNA for RARβ to knock down RARβ mRNA levels in A549 putative CSCs (A549 CD166+CD44+ and A549 CD166+EpCAM+) and A549 putative non- CSCs (A549 CD166-CD44- and A549 CD166-EpCAM-).
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    Human Wharton’s Jelly-Derived Mesenchymal Stem Cells Promote Corneal Epithelial Growth On Polyhydroxyalkanoate For Corneal Regeneration
    (2022-12)
    Mohamed, Mohamed Salih Elnour
    Corneal transplantation has limitations such as graft rejection, disease transmission, and scarcity of tissue supply. Tissue engineering and regenerative medicine technology for future corneal diseases treatment strategies involve the use of safe, commonly available, biocompatible, and biodegradable polymer, together with the biologically active molecules and stem cells. Polyhydroxyalkanoate (PHA) was biosynthesized and characterized by testing the monomer composition and water contact angle. In vitro degradation was explored using lipase enzyme.