Publication: Synthesis And Characterisation Of New Fluorescent Pyrazoline- Piperazinyl Linked Paclitaxel As Prodrugs: Photodynamic Therapy And Anticancer Activity Against Mcf-7 And Mcf-10a Cell Lines
| dc.contributor.author | Luhaibi, Maadh Jumaah Owaid | |
| dc.date.accessioned | 2026-06-19T03:21:40Z | |
| dc.date.available | 2026-06-19T03:21:40Z | |
| dc.date.issued | 2025-05 | |
| dc.description.abstract | This study involves the synthesis of new pendarflour compounds with combination of photodynamic therapy (pdt) and chemotherapy activity against mcf-7 (cancer cells) and mcf-10a (normal cells) which is an effective strategy to treat cancers. A unique photosensitizer (1-17)a (ps)-conjugated paclitaxel (ptx) (1-15)c prodrug has been developed, in which a ps is excited by near-infrared wavelength light to specifically release ptx while generating singlet oxygen (so) to kill cancer cells. The mechanism involves the use of singlet oxygen to cleave aminoacrylate-type linkers in the prodrug. This generates reactive oxygen species (ros) that induce oxidative damage and cell death. Mcf-7 cell death was triggered by ros surpassing the cell’s redox capacity, leading to apoptosis, confirmed by caspase glo-3/7 assays. A paclitaxel (ptx)-based photosensitive conjugate was developed, where light exposure releases intact ptx, combining chemotherapy with immediate pdt action. Pyrazoline (ps) (1-17)a, a key component of the conjugate, was found to be non-toxic and effective in pdt. Uv/vis absorption and fluorescence emission characteristics were evaluated, revealing the stability and reactivity of the photosensitizer. In vitro studies demonstrated that py-l-ptx induced cell death in the presence of light without affecting cells in the dark. | |
| dc.identifier.uri | https://erepo.usm.my/handle/123456789/24423 | |
| dc.language.iso | en | |
| dc.subject | Synthesis Characterisation Fluorescent Pyrazoline- Piperazinyl Linked | |
| dc.title | Synthesis And Characterisation Of New Fluorescent Pyrazoline- Piperazinyl Linked Paclitaxel As Prodrugs: Photodynamic Therapy And Anticancer Activity Against Mcf-7 And Mcf-10a Cell Lines | |
| dc.type | Resource Types::text::thesis::doctoral thesis | |
| dspace.entity.type | Publication | |
| oairecerif.author.affiliation | Universiti Sains Malaysia |