Publication: Development Of Asarone-free Piper Sarmentosum Roxb. Extracts Using Supercritical Carbon Dioxide Pre-treatment And Studies On Their Stability, Cytotoxicity And Enzyme Inhibition
| dc.contributor.author | Hamil, Mohd Shahrul Ridzuan | |
| dc.date.accessioned | 2024-05-15T08:40:16Z | |
| dc.date.available | 2024-05-15T08:40:16Z | |
| dc.date.issued | 2023-09 | |
| dc.description.abstract | Piper sarmentosum Roxburgh is a herbaceous shrub with numerous pharmacological benefits. However, the presence of two toxic phenylpropanoids (α- and β-asarone) has limited the usage of the plant for medicinal purposes. The objectives of the present study include phytochemical analysis of supercritical carbon dioxide (SC-CO2) extract, optimization of SC-CO2 for maximum removal of α-, β- and ɣ-asarone, comparative study between SC-CO2 pre-treated and untreated P. sarmentosum leaf extracts to evaluate their metabolites profiles, bioactivities and stability. The leaves of P. sarmentosum were extracted using SC-CO2 and conventional solvent maceration method to compare the efficiency of asarone extraction. Optimization of asarone extraction was conducted using the Box-Behnken experimental design. SC-CO2 residue was re-extracted using ethanol, 50% ethanol and water, and their chemical and biological profiles were compared with those of leaf material without SC-CO2 pre-treatment. SC-CO2 extraction selectively extracted α-, β- and ɣ-asarone with more than two folds compared to conventional solvent extraction method. Gas chromatography-mass spectrometry (GC-MS) analysis of SC-CO2 extract identified α-asarone (28.18%) as the major component in the extract, followed by γ-asarone (25.70%), phytol (9.66%), asaricin (11.20%) and vitamin E (4.85%). Optimized SC-CO2 condition to maximize the extraction of α-, β- and ɣ-asarone was obtained at pressure = 81.16 bar, temperature = 50.11°C and extraction time = 80.90 min. High performance liquid chromatography (HPLC) analysis of the optimized method showed 13.91% α-asarone, 3.43% β-asarone and 14.95% ɣ-asarone, respectively. SC-CO2 residue of the leaves re-extracted with conventional solvents showed a significant decrease of asarone ranging from 45 to 100% (p<0.001) as compared to their counterpart without SC-CO2 treatment. α-, β- and ɣ-asarone were completely removed in the ethanol and 50% ethanol extracts of the residue, whereas the isomers were absent in water extract. | |
| dc.identifier.uri | https://erepo.usm.my/handle/123456789/19263 | |
| dc.language.iso | en_US | |
| dc.subject | Development Of Asarone-free Piper Sarmentosum Roxb | |
| dc.subject | Extracts Using Supercritical Carbon Dioxide Pre-treatment And Studies | |
| dc.subject | Their Stability | |
| dc.subject | Cytotoxicity And Enzyme Inhibition | |
| dc.subject | Hamil | |
| dc.subject | Mohd Shahrul Ridzuan | |
| dc.subject | Pusat Pengajian Sains Farmasi | |
| dc.title | Development Of Asarone-free Piper Sarmentosum Roxb. Extracts Using Supercritical Carbon Dioxide Pre-treatment And Studies On Their Stability, Cytotoxicity And Enzyme Inhibition | |
| dc.type | Resource Types::text::thesis::doctoral thesis | |
| dspace.entity.type | Publication | |
| oairecerif.author.affiliation | Universiti Sains Malaysia |