Publication:
Construction Of A CRISPR Based Cloning Vector Targeting Ace2

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dc.contributor.authorGunasekaran, Thanes
dc.date.accessioned2026-06-24T02:36:37Z
dc.date.available2026-06-24T02:36:37Z
dc.date.issued2023-09
dc.description.abstractCOVID-19, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in late 2019, poses a significant threat to public health and safety. Efforts to manage the pandemic, including immunizations, have been limited by the emergence of novel SAR CoV-2 variants andthe erosion of immunity. The Protein-Protein interaction network for ACE2 was constructed using Cytoscape 3.10.0 to study the interaction of ACE2 with other genes or proteins involved in the pathogenesis pathway of SARS-Co V -2. Analysis of the ACE2 network showed interaction with nine genes (ACE2, TMPRSS2, CLEC4M, DPP4, ADAM 17, ANEP, CCL2, NPCl, & MEPLA) involved in virus infection process. Besides that, the gene expression analysis of ACE2, TMPRSS2, Arg 1 & IL6 was determined using RT-PCR. The analysis indicated that ACE2 had the highest expression level compared to other three genes in HEK 293 cell line and provided a better understanding of expression of ACE2 in kidney cell line and its related signaling pathway. Apart from that, the construction of an ACE2-knockout CRISPR ·plasmid was successfully executed by designing gRNA and cloning it into a CRISPR plasmid, pX458.
dc.identifier.urihttps://erepo.usm.my/handle/123456789/24462
dc.language.isoen
dc.subjectThanes
dc.subjectGunasekaran
dc.subjectConstruction Of A CRISPR
dc.subjectBased Cloning Vector Targeting Ace2
dc.titleConstruction Of A CRISPR Based Cloning Vector Targeting Ace2
dc.typeResource Types::text::thesis::master thesis
dspace.entity.typePublication
oairecerif.author.affiliationUniversiti Sains Malaysia
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