Publication: Prevalence of hypervirulent klebsiella pneumoniae from patient’s admitted in tertiary centers and their clinical presentation
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Date
2022
Authors
Khairuddin, Aimi
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Abstract
Background: Hypervirulent variant of Klebsiella pneumoniae (hvKp) shows main characteristics of hypermucoviscosity and tendency to cause severe community-acquired infection. Patient infected with this strain may present with more severe illness can lead to fatality. Our study aimed to investigate its prevalence and patient clinical presentation associated with this infection. Methods: A cross-sectional study involving retrospective record review was done with a total of 180 isolates from various clinical specimens were collected from June 2020 till June 2021 in four major hospitals in Kelantan. All isolates were examined by string test for presumptive of hypermucoviscosity (HMKP) and the detection of their virulence genes (rmpA, rmpA2, aerobactin, magA, peg-344, K1, K2) were done by conventional PCR using published primers. HvKp strain is defined as Klebsiella pneumonia strain with presence of hypermucoviscocity and molecular detection of capsular serotyping K1 or K2. Patient’s clinical informations were assessed from medical records and collected data was interpretated using SPSS software version 26. Results: One hundred and eighty K. pneumoniae isolates were obtained from various clinical specimens. String test were positive in 23.8% (n=43) and were identified as hypermucoviscous K. pneumoniae (HMKP). Capsular serotype K1 and K2 were detected in 11.1%(n=20) and 6.1%(n=11) respectively. Therefore, based on definition, prevalence for hvKP was 9.4% (n=17). All virulence-associated genes were statistically significant associated with K1 (p<0.001). There was also significant association between rmpA, rmpA2, aerobactin, peg-344 with K2 (p<0.05) except for magA. Majority of patient presented with respiratory infections. Conclusion: The prevalence of hvKp was found to be 9.4%, lower percentage as compared to others. There were significant association of K1 and K2 with various virulence genes. Respiratory infections were the most common clinical presentation among the hvKp group
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Keywords
HvKP , String test