Publication: Fabrication and molecular dynamics analysis of polydimethylsiloxane antimicrobial nanostructure
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Date
2023-04-01
Authors
Nur Liyana Binti Mohd Shamsuddin
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Abstract
The topographically surface modification using lithography techniques is an alternative approach to create antimicrobial surfaces. The micro/nano structure surfaces mimic the naturally-occur surfaces that are able to repel bacterial attachment or rupture the bacterial cell wall once attached to the surfaces. Nevertheless, the conventional photolithography technique has limits on light diffraction. An electron beam lithography (EBL) the being explored in fabricating the desired micro-and nanofeatures on large areas. This study aimed to fabricate micro/nanostructured of polydimethylsiloxane (PDMS) with antimicrobial properties. The pattern definition process produce the micro/nanohole array on the PMMA/Si mould and the pattern was transferred onto the PDMS using replica moulding (soft lithography) technique. Large-scale Atomic/Molecules Parallel Simulator (LAMMPS) was employed for molecular dynamics (MD) simulation to determine stress-strain response under compression. The adhesion of the Methicillin-resistant Staphylococcus aureus (MRSA) cells was observed and bactericidal efficiency count via the viable plate count method. The PDMS micro/nanostructures arrays of 200 to 300 nm in base diameter and 30-160 nm in height successfully fabricated. The simulated compressive modulus and ultimate compressive strength of PDMS nanostructure was decreased with the increase in simulated temperature. The cell viability diminished by almost 80% and FESEM images showed the cells were deformed and ruptured once attached to the PDMS surface. The topographic features of the PDMS micro/nanostructured surface enhanced the bactericidal properties of the film, which effectively inhibit bacterial attachment and cell proliferation.