Publication: In vitro antioxidative evaluation of plukenetia volubilis aqueous extract in ovarian aging process
| dc.contributor.author | Rusidi, Muhammad Alzam Nafiz | |
| dc.date.accessioned | 2025-12-08T04:21:05Z | |
| dc.date.available | 2025-12-08T04:21:05Z | |
| dc.date.issued | 2025-01 | |
| dc.description.abstract | Ovarian aging is the aging process related to the decline in ovarian cellular function primarily driven by oxidative stress which affects hormonal production, resulting in reduced fertility and hormonal dysfunction in women. Although there are many medications have been developed to increase fertility and reproductive hormones such as clomiphene citrate and gonadotropins, they can cause short- and long-term effects on women. Plukenetia volubilis (Sacha Inchi) is a plant that originated from Northwestern Brazil and Peru and has proven to contain high antioxidant contents such as tocopherols and phenolic compounds. Therefore, our study aims to evaluate the antioxidative potential of P. volubilis aqueous extract in combating oxidative stress during the ovarian aging process using in vitro models. This study assesses the total phenolic content of P. volubilis aqueous extract via total phenolic content (TPC) assay, its radical scavenging ability via DPPH assay, and its protective effects on granulosa cells (COV434) subjected to oxidative stress via MTT assay. Ascorbic acid is used as a standard antioxidant to compare the results. As a result, the TPC assay confirms the presence of significant phenolic content in the extract where the phenolic contents increase as the concentration increases from 0.2 mg/ml to 15 mg/ml. Next, The DPPH assay shows the increase of DPPH radical scavenging with the IC50 value of 2.27 mg/ml. P. volubilis aqueous extract also shows a significant radical scavenging activity when compared to ascorbic acid (p = 0.009). Additionally, the MTT assay reveals the significant protective effects of the extract against hydrogen peroxide-induced oxidative stress in COV434 cells when compared to ascorbic acid (p = 0.089) with the EC50 value of 23.82 mg/ml. This is proven by increasing cell viability when treated with P. volubilis aqueous extract. Overall, the findings successfully underscore the promise of P. volubilis as a natural antioxidant to mitigate oxidative stress-related ovarian aging | |
| dc.identifier.uri | https://erepo.usm.my/handle/123456789/23323 | |
| dc.language.iso | en | |
| dc.subject | - | |
| dc.title | In vitro antioxidative evaluation of plukenetia volubilis aqueous extract in ovarian aging process | |
| dc.type | Resource Types::text::thesis::bachelor thesis | |
| dspace.entity.type | Publication | |
| oairecerif.author.affiliation | Universiti Sains Malaysia |