Publication: Fabrication and characterization of solid and porous dicalcium phosphate dihydrate-coated β-tricalcium phosphate granular
No Thumbnail Available
Date
2023-08-01
Authors
Nur Zulaikha Binti Mohamad Zaidi
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
The aim of this study is to investigate the effect of acidic calcium phosphate solution
concentration and soaking time in fabricating DCPD-coated solid and porous β-TCP granular
as well as its initial cell responses towards bone-like cell. To understand the effect of acidic
calcium phosphate solution concentration and soaking time, the β-TCP granules were
immersed into various concentration of an acidic calcium phosphate solution for 10 mins, 30
mins and 60 mins, respectively.. Then, DCPD-coated solid and porous β-TCP granules were
characterized through several characterization methods such as X-ray Diffraction (XRD),
Fourier Transmission Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM),
Calcium ion release and pH analyses. Based on these analyses showed that that DCPD-coated
porous β-TCP granular produced a large formation of DCPD plate-like crystal coating layer
that helps in stimulating high concentration of calcium ion released while simultaneously
decreasing the pH values of HBSS solution. Meanwhile, to investigate the DCPD-coated solid
and porous β-TCP granules responses towards MC3T3-E1 cells, the solid and porous β-TCP
granules were immersed with 50 mmol/L MCPM- 25 mmol/L H3PO4 solution for 30 mins.
The characterization was similar to the first objective with the addition of qualitative and
quantitative cell analyses. The cell study shows that high amount of DCPD plate-like crystals
formed in DCPD-coated porous β-TCP granules stimulated MC3T3-E1 cell activities
surrounding the DCPD-coated porous β-TCP up to 3 days. Therefore, it was confirmed that
the high amount of DCPD crystals formation and usage of porous β-TCP granules as granular
inner core were accelerated MC3T3-E1 cell activities surrounding the granular.