Publication: Application Of Light-Emitting Diodes On Eurycoma Longifolia Hairy Root Cultures: A Strategy For Enhanced Production Of Antiparasitic Compounds Targeting Blastocystis Sp.
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Date
2025-03
Authors
Abushattal, Mahmoud Ali Khalaf
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Abstract
Eurycoma longifolia (also known as tongkat ali) is a valuable medicinal plant that is frequently used in traditional medicine in malaysia. Its alkaloids and quassinoids reportedly confer antiparasitic effects. The effects of different light-emitting diode (led) spectral treatments on bioactive compounds synthesis, growth characteristics, total carbohydrate and protein content, antioxidant activity, and histological in e. Longifolia hairy root cultures (elhrcs) have not been explored, and the consequent antiparasitic effects on blastocystis sp., a common intestinal protozoan parasite, have not been determined. In this study, elhrcs were irradiated with white, blue, red, blue plus red (1:1) mint green light and dark as control in vitro. The variation in bioactive compound content was assessed by using high-performance liquid chromatography (hplc) were employed to quantify 9-hydroxycanthin-6-one, 9-methoxycanthin-6-one, and eurycomanone. In addition, the dichloromethane (dcm) crude extract was studied through the isolation, purification, and structural elucidation processes, resulting in the discovery of two known compounds, namely canthin-6-one and 9-methoxycanthin-6-one.
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Plant tissue culture