Publication: Development of a leakage-free printed circuit board (pcb) based biosensor for dna sensing
| datacite.subject.fos | oecd::Engineering and technology::Electrical engineering, Electronic engineering, Information engineering | |
| dc.contributor.author | Norshah Rizal Bin Ali @ Hasim | |
| dc.date.accessioned | 2025-05-08T03:11:49Z | |
| dc.date.available | 2025-05-08T03:11:49Z | |
| dc.date.issued | 2023-10-01 | |
| dc.description.abstract | This research has developed a leakage-free and disposable deoxyribonucleic acid (DNA) fingerprinting biochip, utilising amperometric detection on copper-clad electrodes of FR-4 printed circuit board (PCB), with polydimethylsiloxane (PDMS) as the separation microchannel. Fluid leakage is a significant challenge when building a Lab-on-PCB (LOP) microfluidic device. Copper-clad electrodes simplify fabrication and make it disposable. Nevertheless, a non-inert copper requires stable biological buffers to stabilise the DNA. Redox properties investigation of nucleotides and nucleobases as the target detection molecules are vital for improving biochip transduction and detection resolution. Hence, the research objectives have been set up to solve the leakage issue, the investigation of biological buffers, and the analysis of target detection molecules in DNA. A preventative layer from photocurable diacrylate bisphenol-A polymer (DABA) provided irreversible bonding between PDMS and the PCB substrates. It was the first DABA application which has never been tested in dentistry or biomedical applications. Tensile tests on PCB-DABA-PDMS revealed bond breakages at the DABA-PCB interface, with an average tensile strength of 287.36 kPa and a standard deviation of 23.79 kPa. Meanwhile, the leakage test indicated that the microchannel could resist more than 189 kPa pressures, which is acceptable for this application. Cyclic voltammetry (CV) experiments on the copper electrode with ethylenediaminetetraacetic acid (EDTA) and 2-(N-morpholino)ethanesulfonic acid (MES) buffers showed that deoxyguanosine triphosphate (dGTP) molecules undergo irreversible oxidation potentials at 0.9 and 1.245 V, respectively. The experiments also confirm that the dGTP was stable inside the buffers and sensitive to the obtained potentials. DNA analysis experiments on single-band DNA (PCR amplicon) and multiple-band (1kbp DNA ladders) revealed that the proposed design could accurately separate and detect DNA fragments at an electric field strength of 20V/cm with low background noise. The first findings show that DABA is biocompatible and biologically inert to DNA samples, where the new technique efficiently closes the device, preventing liquid leakage from the sensor’s grid. Secondly, DNA stability with copper electrodes could also be achieved electrochemically within EDTA and MES buffers. Finally, the detecting target molecule employing guanine nucleotides offers a good current detection even with a small DNA sample. The discoveries paved the way for the development of completely disposable biosensing devices. | |
| dc.identifier.uri | https://erepo.usm.my/handle/123456789/21550 | |
| dc.language.iso | en | |
| dc.title | Development of a leakage-free printed circuit board (pcb) based biosensor for dna sensing | |
| dc.type | Resource Types::text::thesis::doctoral thesis | |
| dspace.entity.type | Publication | |
| oairecerif.author.affiliation | Universiti Sains Malaysia |