Publication:
Polarographic determination of ascorbic acid in roselle juice samples.

dc.contributor.authorOmar, Rosila
dc.date.accessioned2024-04-29T08:24:09Z
dc.date.available2024-04-29T08:24:09Z
dc.date.issued2015
dc.description.abstractAscorbic acid which is also known as vitamin C cannot be synthesised in human body. Hence, it is important to get the vitamin C from other source such as roseile juice. The recommended daily intake of vitamin C is 60 mg/day. Polarography technique has been used to determine the concentration of vitamin C in the roseile juice samples. The parameters for polarography technique for ascorbic acid determination are as follows: start potential: -0.0499 V, end potential: 0.1299 V, deposition potential: 0.55 V, deposition time: 15 s, equilibrium time: 10 s, voltage step: 0.005951 V, voltage step time: 0.6 s, sweep rate: 0.0099 V/s, pulse amplitude: 0.05 V, and pulse time: 0.04 s. The electrolytes used were 10 ml deionised water and 1 ml acetate buffer with pH of 4.64. The peak potential of ascorbic acid was found at 0.063 V. From five commercial roseile juice samples, three of them give a positive result on the ascorbic acid content. In the analysis of raw samples, it was found that the leaves have higher levels of vitamin C compared to calyces. As the conclusion, polarography technique was a successful applied for the determination of ascorbic acid in commercial roseile juice samples and raw samples such as roseile calyces and leaves.
dc.identifier.urihttps://erepo.usm.my/handle/123456789/19040
dc.language.isoother
dc.titlePolarographic determination of ascorbic acid in roselle juice samples.
dc.typeResource Types::text::report::research report
dspace.entity.typePublication
oairecerif.author.affiliation#PLACEHOLDER_PARENT_METADATA_VALUE#
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