Publication: Comparison of oxidative stress biomarkers level in tears and serum of patients with alzheimer’s disease
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Date
2021
Authors
Juan, Juni Eyat Ju Ying Ap
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Abstract
Introduction: Oxidative stress plays an important role in Alzheimer’s disease (AD). Objective: Our objective is to compare glutathione peroxidase (GPx), superoxide dismutase (SOD) enzyme activities and malondialdehyde (MDA) level in tears and serum between AD and controls. This study also aims to identify the correlation between GPx, SOD and MDA in tears and serum among AD patients. Methods: A cross-sectional study was conducted between November 2017 to December 2019 involving Alzheimer patients who attended psychiatric clinic and controls attended ophthalmology clinic in Hospital Universiti Sains Malaysia (HUSM). Tears samples were collected using Schirmer paper while blood samples were collected using plain tube bottle. Laboratory analysis was performed using commercially available oxidative stress markers kits. Statistically analysis was done using Statistical Package for the Social Science (SPSS Inc Version 26). Results: A total of 50 patients (25 Alzheimer’s disease and 25 controls) were recruited. Mean superoxide dismutase (p=0.008) and glutathione peroxidase (p=0.003) in tears were significantly lower in Alzheimer patients compared to controls with a mean difference of 0.02 (95% confident interval 0.01,0.03) and 437.02 (95% confident interval 171.22,702.83) respectively while mean superoxide dismutase in serum was significantly higher (p=0.045) in Alzheimer patients with mean difference of -0.01(95%confident interval -0.03,-0.00)] as compared to control, after adjusted for age, smoking and medical problems. There was a very weak correlation in superoxide dismutase, glutathione peroxidase and malondialdehyde between serum and tears in Alzheimer patients. Conclusion: This study showed significantly low superoxide dismutase and glutathione peroxidase activities in tears while high superoxide dismutase activity in serum among Alzheimer patients as compared to controls.
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Keywords
Oxidative stress , superoxide dismutase