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Determination of group B streptococcus (gbs) serotypes, its virulenece genes and patient’s clinical presentation among non-pregnant adults in Kelantan

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Date
2021
Authors
Sulung @ Ab Hadi, Faridah
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Research Projects
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Abstract
Background: GBS infection among non-pregnant adults is emerging nowadays. The severity of the disease caused by GBS depends on the serotype and presence of virulence genes in the particular strains. This study aimed to determine the distribution of GBS serotypes and virulence genes and to know the association of GBS serotype with the clinical disease. Methods: A cross-sectional study involving retrospective record review was done involving a total of 75 GBS isolates collected from October 2018 till October 2019 in two major hospitals in Kelantan (East coast of peninsula Malaysia). Those isolates were from various clinical samples of non-pregnant adults > 18 years old (excluding vaginal swab). Identification of all isolates serotype (serotype Ia, Ib, II until IX) and their virulence genes (scpB, lmb, bca, bac, rib, cylE, and hylE) were done using conventional PCR. The antibiotic susceptibility testing was traced from The BIOMIC V3, Microbiology System. Clinical information of patient was assessed from medical records and were analysed and presented as tables and figures. Background: GBS infection among non-pregnant adults is emerging nowadays. The severity of the disease caused by GBS depends on the serotype and presence of virulence genes in the particular strains. This study aimed to determine the distribution of GBS serotypes and virulence genes and to know the association of GBS serotype with the clinical disease. Methods: A cross-sectional study involving retrospective record review was done involving a total of 75 GBS isolates collected from October 2018 till October 2019 in two major hospitals in Kelantan (East coast of peninsula Malaysia). Those isolates were from various clinical samples of non-pregnant adults > 18 years old (excluding vaginal swab). Identification of all isolates serotype (serotype Ia, Ib, II until IX) and their virulence genes (scpB, lmb, bca, bac, rib, cylE, and hylE) were done using conventional PCR. The antibiotic susceptibility testing was traced from The BIOMIC V3, Microbiology System. Clinical information of patient was assessed from medical records and were analysed and presented as tables and figures.
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Keywords
GBS , non-pregnant
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